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RecombinantRabMAb

Recombinant Anti-RNF7 antibody [EPR12001] (ab181986)

Reviews (1) Submit a question References (5)
Western blot - Anti-RNF7 antibody [EPR12001] (ab181986)
  • Immunocytochemistry/ Immunofluorescence - Anti-RNF7 antibody [EPR12001] (ab181986)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF7 antibody [EPR12001] (ab181986)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF7 antibody [EPR12001] (ab181986)
  • Western blot - Anti-RNF7 antibody [EPR12001] (ab181986)
  • Anti-RNF7 antibody [EPR12001] (ab181986)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12001] to RNF7
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-RNF7 antibody [EPR12001]
    See all RNF7 primary antibodies

  • Description

    Rabbit monoclonal [EPR12001] to RNF7

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2, Mouse kidney or Mouse heart lysate, Human transitional cell carcinoma of bladder or Mouse liver tissue, HepG2 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab181986 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

For antigen retrieval heat up to 98 °C, below boiling, and then let cool for 10-20 min.
WB
1/1000 - 1/10000. Predicted molecular weight: 13 kDa.
ICC/IF (1)
1/100.
Notes
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

For antigen retrieval heat up to 98 °C, below boiling, and then let cool for 10-20 min.
WB
1/1000 - 1/10000. Predicted molecular weight: 13 kDa.
ICC/IF
1/100.

Target

  • Function

    Probable component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. Promotes the neddylation of CUL5 via its interaction with UBE2F. May play a role in protecting cells from apoptosis induced by redox agents.

  • Tissue specificity

    Expressed in heart, liver, skeletal muscle and pancreas. At very low levels expressed in brain, placenta and lung.

  • Pathway

    Protein modification; protein ubiquitination.

  • Sequence similarities

    Belongs to the RING-box family.
    Contains 1 RING-type zinc finger.

  • Domain

    The RING-type zinc finger domain is essential for ubiquitin ligase activity. It coordinates an additional third zinc ion.

  • Post-translational
    modifications

    Phosphorylation by CK2 is required for efficient degradation of NFKBIA and CDKN1B.

  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • CKBBP 1 antibody
    • CKBBP1 antibody
    • CKII beta binding protein 1 antibody
    • CKII beta-binding protein 1 antibody
    • Rbx 2 antibody
    • Rbx2 antibody
    • RBX2_HUMAN antibody
    • Regulator of cullins 2 antibody
    • RING box protein 2 antibody
    • RING finger protein 7 antibody
    • RING-box protein 2 antibody
    • RNF 7 antibody
    • RNF7 antibody
    • ROC 2 antibody
    • ROC2 antibody
    • SAG antibody
    • Sensitive to apoptosis gene antibody
    • Sensitive to apoptosis gene protein antibody
    • Zinc RING finger protein SAG antibody
    see all

Images

  • Western blot - Anti-RNF7 antibody [EPR12001] (ab181986)
    Western blot - Anti-RNF7 antibody [EPR12001] (ab181986)
    All lanes : Anti-RNF7 antibody [EPR12001] (ab181986) at 1/5000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Mouse kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 13 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-RNF7 antibody [EPR12001] (ab181986)
    Immunocytochemistry/ Immunofluorescence - Anti-RNF7 antibody [EPR12001] (ab181986)

    Immunofluorescence analysis of HepG2 cells (fixative 4% paraformaldehyde) labeling RNF7 with ab181986 at a 1/100 dilution.Goat anti rabbit IgG (Dylight 555) secondary used at a 1/200 diution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF7 antibody [EPR12001] (ab181986)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF7 antibody [EPR12001] (ab181986)

    Immunohistochemical analysis of paraffin embedded Mouse liver tissue labeling RNF7 with ab181986 at a 1/50 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF7 antibody [EPR12001] (ab181986)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF7 antibody [EPR12001] (ab181986)

    Immunohistochemical analysis of paraffin embedded Human transitional cell carcinoma of bladder  tissue labeling RNF7 with
    ab181986 at a 1/50 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-RNF7 antibody [EPR12001] (ab181986)
    Western blot - Anti-RNF7 antibody [EPR12001] (ab181986)
    Anti-RNF7 antibody [EPR12001] (ab181986) at 1/5000 dilution + HepG2 lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 13 kDa

  • Anti-RNF7 antibody [EPR12001] (ab181986)
    Anti-RNF7 antibody [EPR12001] (ab181986)

References (5)

Publishing research using ab181986? Please let us know so that we can cite the reference in this datasheet.

ab181986 has been referenced in 5 publications.

  • Xu D  et al. Cullin 2-RBX1 E3 ligase and USP2 regulate antithrombin ubiquitination and stability. FASEB J 35:e21800 (2021). PubMed: 34324733
  • Kabir S  et al. The CUL5 ubiquitin ligase complex mediates resistance to CDK9 and MCL1 inhibitors in lung cancer cells. Elife 8:N/A (2019). PubMed: 31294695
  • Zeng H  et al. Genome-wide CRISPR screening reveals genetic modifiers of mutant EGFR dependence in human NSCLC. Elife 8:N/A (2019). PubMed: 31741433
  • Hino K  et al. Comparative Analysis of cul5 and rbx2 Expression in the Developing and Adult Murine Brain and Their Essentiality During Mouse Embryogenesis. Dev Dyn 247:1227-1236 (2018). PubMed: 30269386
  • Xiao Y  et al. RNF7 knockdown inhibits prostate cancer tumorigenesis by inactivation of ERK1/2 pathway. Sci Rep 7:43683 (2017). WB . PubMed: 28252001

Customer reviews and Q&As

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Immunocytochemistry/ Immunofluorescence abreview for Anti-RNF7 antibody [EPR12001]

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X-100
Specification
HeLa
Fixative
Paraformaldehyde
Read More

DR. Kirk Mcmanus

Verified customer

Submitted Jan 04 2018

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