For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    rnf7-antibody-epr12001-ab181986.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cell Biology Apoptosis Intracellular Associated Proteins
Share by email
RecombinantRabMAb

Recombinant Anti-RNF7 antibody [EPR12001] (ab181986)

  • Datasheet
  • Certificate of Compliance
Reviews (1) Submit a question References (5)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-RNF7 antibody [EPR12001] (ab181986)
  • Immunocytochemistry/ Immunofluorescence - Anti-RNF7 antibody [EPR12001] (ab181986)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF7 antibody [EPR12001] (ab181986)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF7 antibody [EPR12001] (ab181986)
  • Western blot - Anti-RNF7 antibody [EPR12001] (ab181986)
  • Anti-RNF7 antibody [EPR12001] (ab181986)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12001] to RNF7
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Primary
Product image
Anti-Bik antibody (ab52182)

View more associated products

Overview

  • Product name

    Anti-RNF7 antibody [EPR12001]
    See all RNF7 primary antibodies
  • Description

    Rabbit monoclonal [EPR12001] to RNF7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2, Mouse kidney or Mouse heart lysate, Human transitional cell carcinoma of bladder or Mouse liver tissue, HepG2 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12001
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Associated Proteins
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • RING Finger E3 Ligase
    • Epigenetics and Nuclear Signaling
    • Ubiquitin & Ubiquitin Like Modifiers
    • E3 Ubiquitin Ligases
    • Epigenetics and Nuclear Signaling
    • Ubiquitin & Ubiquitin Like Modifiers
    • Ubiquitin like Modifiers
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress

Associated products

  • Alternative Versions

    • Anti-RNF7 antibody [EPR12001] - BSA and Azide free (ab250553)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Mouse kidney normal tissue lysate - total protein (ab29305)
    • Mouse heart normal tissue lysate - total protein (ab30291)
  • Related Products

    • Recombinant Human RNF7 protein (denatured) (ab171587)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab181986 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

For antigen retrieval heat up to 98 °C, below boiling, and then let cool for 10-20 min.

WB
1/1000 - 1/10000. Predicted molecular weight: 13 kDa.
ICC/IF (1)
1/100.
Notes
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

For antigen retrieval heat up to 98 °C, below boiling, and then let cool for 10-20 min.

WB
1/1000 - 1/10000. Predicted molecular weight: 13 kDa.
ICC/IF
1/100.

Target

  • Function

    Probable component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. Promotes the neddylation of CUL5 via its interaction with UBE2F. May play a role in protecting cells from apoptosis induced by redox agents.
  • Tissue specificity

    Expressed in heart, liver, skeletal muscle and pancreas. At very low levels expressed in brain, placenta and lung.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the RING-box family.
    Contains 1 RING-type zinc finger.
  • Domain

    The RING-type zinc finger domain is essential for ubiquitin ligase activity. It coordinates an additional third zinc ion.
  • Post-translational
    modifications

    Phosphorylation by CK2 is required for efficient degradation of NFKBIA and CDKN1B.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Target information above from: UniProt accession Q9UBF6 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9616 Human
    • Entrez Gene: 19823 Mouse
    • Entrez Gene: 300948 Rat
    • Omim: 603863 Human
    • SwissProt: Q9UBF6 Human
    • SwissProt: Q9WTZ1 Mouse
    • SwissProt: D3Z8P1 Rat
    • Unigene: 134623 Human
    • Unigene: 28235 Mouse
    see all
  • Alternative names

    • CKBBP 1 antibody
    • CKBBP1 antibody
    • CKII beta binding protein 1 antibody
    • CKII beta-binding protein 1 antibody
    • Rbx 2 antibody
    • Rbx2 antibody
    • RBX2_HUMAN antibody
    • Regulator of cullins 2 antibody
    • RING box protein 2 antibody
    • RING finger protein 7 antibody
    • RING-box protein 2 antibody
    • RNF 7 antibody
    • RNF7 antibody
    • ROC 2 antibody
    • ROC2 antibody
    • SAG antibody
    • Sensitive to apoptosis gene antibody
    • Sensitive to apoptosis gene protein antibody
    • Zinc RING finger protein SAG antibody
    see all

Images

  • Western blot - Anti-RNF7 antibody [EPR12001] (ab181986)
    Western blot - Anti-RNF7 antibody [EPR12001] (ab181986)
    All lanes : Anti-RNF7 antibody [EPR12001] (ab181986) at 1/5000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Mouse kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 13 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-RNF7 antibody [EPR12001] (ab181986)
    Immunocytochemistry/ Immunofluorescence - Anti-RNF7 antibody [EPR12001] (ab181986)

    Immunofluorescence analysis of HepG2 cells (fixative 4% paraformaldehyde) labeling RNF7 with ab181986 at a 1/100 dilution.Goat anti rabbit IgG (Dylight 555) secondary used at a 1/200 diution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF7 antibody [EPR12001] (ab181986)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF7 antibody [EPR12001] (ab181986)

    Immunohistochemical analysis of paraffin embedded Mouse liver tissue labeling RNF7 with ab181986 at a 1/50 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF7 antibody [EPR12001] (ab181986)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF7 antibody [EPR12001] (ab181986)

    Immunohistochemical analysis of paraffin embedded Human transitional cell carcinoma of bladder  tissue labeling RNF7 with
    ab181986 at a 1/50 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-RNF7 antibody [EPR12001] (ab181986)
    Western blot - Anti-RNF7 antibody [EPR12001] (ab181986)
    Anti-RNF7 antibody [EPR12001] (ab181986) at 1/5000 dilution + HepG2 lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 13 kDa

  • Anti-RNF7 antibody [EPR12001] (ab181986)
    Anti-RNF7 antibody [EPR12001] (ab181986)

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (5)

Publishing research using ab181986? Please let us know so that we can cite the reference in this datasheet.

ab181986 has been referenced in 5 publications.

  • Xu D  et al. Cullin 2-RBX1 E3 ligase and USP2 regulate antithrombin ubiquitination and stability. FASEB J 35:e21800 (2021). PubMed: 34324733
  • Kabir S  et al. The CUL5 ubiquitin ligase complex mediates resistance to CDK9 and MCL1 inhibitors in lung cancer cells. Elife 8:N/A (2019). PubMed: 31294695
  • Zeng H  et al. Genome-wide CRISPR screening reveals genetic modifiers of mutant EGFR dependence in human NSCLC. Elife 8:N/A (2019). PubMed: 31741433
  • Hino K  et al. Comparative Analysis of cul5 and rbx2 Expression in the Developing and Adult Murine Brain and Their Essentiality During Mouse Embryogenesis. Dev Dyn 247:1227-1236 (2018). PubMed: 30269386
  • Xiao Y  et al. RNF7 knockdown inhibits prostate cancer tumorigenesis by inactivation of ERK1/2 pathway. Sci Rep 7:43683 (2017). WB . PubMed: 28252001

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Immunocytochemistry/ Immunofluorescence abreview for Anti-RNF7 antibody [EPR12001]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X-100
Specification
HeLa
Fixative
Paraformaldehyde
Read More

DR. Kirk Mcmanus

Verified customer

Submitted Jan 04 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2022 Abcam plc. All rights reserved.