Recombinant Anti-RNF7 antibody [EPR12001] (ab181986)
![Western blot - Anti-RNF7 antibody [EPR12001] (ab181986)](https://www.abcam.com/ps/products/181/ab181986/Images/ab181986-215434-anti-rnf7-antibody-epr12001-western-blot.jpg "Western blot - Anti-RNF7 antibody [EPR12001] (ab181986)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12001] to RNF7
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
-
Product name
Anti-RNF7 antibody [EPR12001]
See all RNF7 primary antibodies -
Description
Rabbit monoclonal [EPR12001] to RNF7 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
-
Immunogen
This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- HepG2, Mouse kidney or Mouse heart lysate, Human transitional cell carcinoma of bladder or Mouse liver tissue, HepG2 cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12001 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181986 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
For antigen retrieval heat up to 98 °C, below boiling, and then let cool for 10-20 min. |
|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 13 kDa.
|
|
| ICC/IF | (1) |
1/100.
|
| Notes |
|---|
|
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. For antigen retrieval heat up to 98 °C, below boiling, and then let cool for 10-20 min. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 13 kDa. |
|
ICC/IF
1/100. |
Target
-
Function
Probable component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. Promotes the neddylation of CUL5 via its interaction with UBE2F. May play a role in protecting cells from apoptosis induced by redox agents. -
Tissue specificity
Expressed in heart, liver, skeletal muscle and pancreas. At very low levels expressed in brain, placenta and lung. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the RING-box family.
Contains 1 RING-type zinc finger. -
Domain
The RING-type zinc finger domain is essential for ubiquitin ligase activity. It coordinates an additional third zinc ion. -
Post-translational
modificationsPhosphorylation by CK2 is required for efficient degradation of NFKBIA and CDKN1B. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 9616 Human
- Entrez Gene: 19823 Mouse
- Entrez Gene: 300948 Rat
- Omim: 603863 Human
- SwissProt: Q9UBF6 Human
- SwissProt: Q9WTZ1 Mouse
- SwissProt: D3Z8P1 Rat
- Unigene: 134623 Human
see all -
Alternative names
- CKBBP 1 antibody
- CKBBP1 antibody
- CKII beta binding protein 1 antibody
see all
Images
-
Western blot - Anti-RNF7 antibody [EPR12001] (ab181986)All lanes : Anti-RNF7 antibody [EPR12001] (ab181986) at 1/5000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Mouse kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 13 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-RNF7 antibody [EPR12001] (ab181986)](https://www.abcam.com/ps/products/181/ab181986/Images/ab181986-215438-anti-rnf7-antibody-epr12001-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-RNF7 antibody [EPR12001] (ab181986)Immunofluorescence analysis of HepG2 cells (fixative 4% paraformaldehyde) labeling RNF7 with ab181986 at a 1/100 dilution.Goat anti rabbit IgG (Dylight 555) secondary used at a 1/200 diution.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF7 antibody [EPR12001] (ab181986)](https://www.abcam.com/ps/products/181/ab181986/Images/ab181986-215437-anti-rnf7-antibody-epr12001-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF7 antibody [EPR12001] (ab181986)Immunohistochemical analysis of paraffin embedded Mouse liver tissue labeling RNF7 with ab181986 at a 1/50 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF7 antibody [EPR12001] (ab181986)](https://www.abcam.com/ps/products/181/ab181986/Images/ab181986-215436-anti-rnf7-antibody-epr12001-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF7 antibody [EPR12001] (ab181986)Immunohistochemical analysis of paraffin embedded Human transitional cell carcinoma of bladder tissue labeling RNF7 with
ab181986 at a 1/50 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
![Western blot - Anti-RNF7 antibody [EPR12001] (ab181986)](https://www.abcam.com/ps/products/181/ab181986/Images/ab181986-215433-anti-rnf7-antibody-epr12001-western-blot.jpg)
Western blot - Anti-RNF7 antibody [EPR12001] (ab181986)Anti-RNF7 antibody [EPR12001] (ab181986) at 1/5000 dilution + HepG2 lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 13 kDa -
![Anti-RNF7 antibody [EPR12001] (ab181986)](https://www.abcam.com/ps/products/181/ab181986/Images/ab181986-6-benefits-of-recombinant-antibodies.png)
Anti-RNF7 antibody [EPR12001] (ab181986)
Protocols
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (4)
ab181986 has been referenced in 4 publications.
- Kabir S et al. The CUL5 ubiquitin ligase complex mediates resistance to CDK9 and MCL1 inhibitors in lung cancer cells. Elife 8:N/A (2019). PubMed: 31294695
- Zeng H et al. Genome-wide CRISPR screening reveals genetic modifiers of mutant EGFR dependence in human NSCLC. Elife 8:N/A (2019). PubMed: 31741433
- Hino K et al. Comparative Analysis of cul5 and rbx2 Expression in the Developing and Adult Murine Brain and Their Essentiality During Mouse Embryogenesis. Dev Dyn 247:1227-1236 (2018). PubMed: 30269386
- Xiao Y et al. RNF7 knockdown inhibits prostate cancer tumorigenesis by inactivation of ERK1/2 pathway. Sci Rep 7:43683 (2017). WB . PubMed: 28252001
