Anti-RNF7 antibody [EPR12001] (ab181986)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-RNF7 antibody [EPR12001]
See all RNF7 primary antibodies -
DescriptionRabbit monoclonal [EPR12001] to RNF7
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human RNF7 aa 100 to the C-terminus. The exact sequence is proprietary.
Database link: Q9UBF6 -
Positive control
- HepG2, Mouse kidney or Mouse heart lysate, Human transitional cell carcinoma of bladder or Mouse liver tissue, HepG2 cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, 59% PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR12001
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IsotypeIgG
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Research areas
Associated products
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab181986 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50. For antigen retrieval heat up to 98 °C, below boiling, and then let cool for 10-20 min. |
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| WB | 1/1000 - 1/10000. Predicted molecular weight: 13 kDa. | |
| ICC/IF | 1/100. |
Target
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FunctionProbable component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. Promotes the neddylation of CUL5 via its interaction with UBE2F. May play a role in protecting cells from apoptosis induced by redox agents.
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Tissue specificityExpressed in heart, liver, skeletal muscle and pancreas. At very low levels expressed in brain, placenta and lung.
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PathwayProtein modification; protein ubiquitination.
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Sequence similaritiesBelongs to the RING-box family.
Contains 1 RING-type zinc finger. -
DomainThe RING-type zinc finger domain is essential for ubiquitin ligase activity. It coordinates an additional third zinc ion.
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Post-translational
modificationsPhosphorylation by CK2 is required for efficient degradation of NFKBIA and CDKN1B. -
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
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Database links
- Entrez Gene: 9616 Human
- Entrez Gene: 19823 Mouse
- Entrez Gene: 300948 Rat
- Omim: 603863 Human
- SwissProt: Q9UBF6 Human
- SwissProt: Q9WTZ1 Mouse
- SwissProt: D3Z8P1 Rat
- Unigene: 134623 Human
see all -
Alternative names
- CKBBP 1 antibody
- CKBBP1 antibody
- CKII beta binding protein 1 antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-RNF7 antibody [EPR12001] (ab181986)](https://www.abcam.com/ps/products/181/ab181986/Images/ab181986-215438-ab181986if1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-RNF7 antibody [EPR12001] (ab181986)Immunofluorescence analysis of HepG2 cells (fixative 4% paraformaldehyde) labeling RNF7 with ab181986 at a 1/100 dilution.Goat anti rabbit IgG (Dylight 555) secondary used at a 1/200 diution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF7 antibody [EPR12001] (ab181986)](https://www.abcam.com/ps/products/181/ab181986/Images/ab181986-215437-ab181986ihc2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF7 antibody [EPR12001] (ab181986)Immunohistochemical analysis of paraffin embedded Mouse liver tissue labeling RNF7 with ab181986 at a 1/50 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF7 antibody [EPR12001] (ab181986)](https://www.abcam.com/ps/products/181/ab181986/Images/ab181986-215436-ab181986ihc1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF7 antibody [EPR12001] (ab181986)Immunohistochemical analysis of paraffin embedded Human transitional cell carcinoma of bladder tissue labeling RNF7 with
ab181986 at a 1/50 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin. -
![Western blot - Anti-RNF7 antibody [EPR12001] (ab181986)](https://www.abcam.com/ps/products/181/ab181986/Images/ab181986-215434-ab181986wb2.jpg)
Western blot - Anti-RNF7 antibody [EPR12001] (ab181986)All lanes : Anti-RNF7 antibody [EPR12001] (ab181986) at 1/5000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Mouse kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 13 kDa -
![Western blot - Anti-RNF7 antibody [EPR12001] (ab181986)](https://www.abcam.com/ps/products/181/ab181986/Images/ab181986-215433-ab181986wb1.jpg)
Western blot - Anti-RNF7 antibody [EPR12001] (ab181986)Anti-RNF7 antibody [EPR12001] (ab181986) at 1/5000 dilution + HepG2 lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 13 kDa
Protocols
References
This product has been referenced in:
- Xiao Y et al. RNF7 knockdown inhibits prostate cancer tumorigenesis by inactivation of ERK1/2 pathway. Sci Rep 7:43683 (2017). WB . Read more (PubMed: 28252001) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"