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RecombinantRabMAb

Recombinant Anti-RNF8 antibody [EPR6311(2)] - BSA and Azide free (ab248198)

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Western blot - Anti-RNF8 antibody [EPR6311(2)] - BSA and Azide free (ab248198)
  • Anti-RNF8 antibody [EPR6311(2)] - BSA and Azide free (ab248198)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6311(2)] to RNF8 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-RNF8 antibody [EPR6311(2)] - BSA and Azide free
    See all RNF8 primary antibodies

  • Description

    Rabbit monoclonal [EPR6311(2)] to RNF8 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248198 is the carrier-free version of ab128872.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab248198 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).
Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).
Application notes
Is unsuitable for IHC-P or IP.

Target

  • Function

    E3 ubiquitin-protein ligase required for assembly of repair proteins to sites of DNA damage. Catalyzes the 'Lys-63'-linked ubiquitination of histone H2A and H2AX. Following DNA double-strand breaks (DSBs), it is recruited to the sites of damage by ATM-phosphorylated MDC1, mediates the ubiquitination of histones H2A and H2AX, thereby promoting the formation of TP53BP1 and BRCA1 ionizing radiation-induced foci (IRIF). Promotes the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBE2N/UBC13. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation. Enforces the G2/M DNA damage checkpoint. Controls the recruitment of UIMC1-BRCC3 (RAP80-BRCC36) and PAXIP1/PTIP to DNA damage sites following DNA double-strand breaks (DSBs). Ubiquitination of histone H2A requires UBE2N but not MMS2 (UBE2V2). May also ubiquitinate histone H2B. Catalyzes the 'Lys-63'-linked ubiquitination of PCNA. May be required for proper exit from mitosis after spindle checkpoint activation and may regulate cytokinesis. May play a role in the regulation of RXRA-mediated transcriptional activity. Not involved in RXRA ubiquitination by UBE2E2.

  • Tissue specificity

    Ubiquitous. In fetal tissues, highest expression in brain, thymus and liver. In adult tissues, highest levels in brain and testis, lowest levels in peripheral blood cells.

  • Pathway

    Protein modification; protein ubiquitination.

  • Sequence similarities

    Belongs to the RNF8 family.
    Contains 1 FHA domain.
    Contains 1 RING-type zinc finger.

  • Developmental stage

    Low levels at the G1-S boundary increase in intensity during S phase and until the end of the G2 phase. Abruptly decreases in late mitosis (at protein level). Barely detectable in anaphase.

  • Domain

    The FHA domain specifically recognizes and binds ATM-phosphorylated MDC1 and Thr-4827 phosphorylated HERC2.

  • Post-translational
    modifications

    Autoubiquitinated through 'Lys-48' and 'Lys-63' of ubiquitin. 'Lys-63' polyubiquitination is mediated by UBE2N. 'Lys-29'-type polyubiquitination is also observed, but it doesn't require its own functional RING-type zinc finger.

  • Cellular localization

    Nucleus. Midbody. Following DNA double-strand breaks, recruited to the sites of damage. During prophase, concomitant with nuclear envelope breakdown, localizes throughout the cell, with a dotted pattern. In telophase, again in the nucleus and also with a discrete dotted pattern in the cytoplasm. In late telophase and during cytokinesis, localizes in the midbody of the tubulin bridge joining the daughter cells. Does not seem to be associated with condensed chromosomes at any time during the cell cycle.
  • Information by UniProt

  • Database links

    • Alternative names

      • C3HC4 type zinc finger protein antibody
      • E3 ubiquitin protein ligase RNF8 antibody
      • E3 ubiquitin-protein ligase RNF8 antibody
      • FLJ12013 antibody
      • KIAA0646 antibody
      • Ring finger protein (C3HC4 type) 8 antibody
      • RING finger protein 8 antibody
      • Ring finger protein 8, E3 ubiquitin protein ligase antibody
      • RNF 8 antibody
      • RNF8 antibody
      • RNF8_HUMAN antibody
      • UBC13/UEV interacting ring finger protein antibody
      see all

    Images

    • Western blot - Anti-RNF8 antibody [EPR6311(2)] - BSA and Azide free (ab248198)
      Western blot - Anti-RNF8 antibody [EPR6311(2)] - BSA and Azide free (ab248198)
      All lanes : Anti-RNF8 antibody [EPR6311(2)] (ab128872) at 1/1000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : Human fetal thymus lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 55 kDa



      This data was developed using ab128872, the same antibody clone in a different buffer formulation.

       

    • Anti-RNF8 antibody [EPR6311(2)] - BSA and Azide free (ab248198)
      Anti-RNF8 antibody [EPR6311(2)] - BSA and Azide free (ab248198)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab248198? Please let us know so that we can cite the reference in this datasheet.

    ab248198 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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