Overview

  • Product name

    Anti-RNF8 antibody [EPR6312(2)]
    See all RNF8 primary antibodies
  • Description

    Rabbit monoclonal [EPR6312(2)] to RNF8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human RNF8 aa 400-500. The exact sequence is proprietary.

  • Positive control

    • Human fetal brain, fetal thymus, and fetal kidney lysates.
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab131221 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function

      E3 ubiquitin-protein ligase required for assembly of repair proteins to sites of DNA damage. Catalyzes the 'Lys-63'-linked ubiquitination of histone H2A and H2AX. Following DNA double-strand breaks (DSBs), it is recruited to the sites of damage by ATM-phosphorylated MDC1, mediates the ubiquitination of histones H2A and H2AX, thereby promoting the formation of TP53BP1 and BRCA1 ionizing radiation-induced foci (IRIF). Promotes the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBE2N/UBC13. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation. Enforces the G2/M DNA damage checkpoint. Controls the recruitment of UIMC1-BRCC3 (RAP80-BRCC36) and PAXIP1/PTIP to DNA damage sites following DNA double-strand breaks (DSBs). Ubiquitination of histone H2A requires UBE2N but not MMS2 (UBE2V2). May also ubiquitinate histone H2B. Catalyzes the 'Lys-63'-linked ubiquitination of PCNA. May be required for proper exit from mitosis after spindle checkpoint activation and may regulate cytokinesis. May play a role in the regulation of RXRA-mediated transcriptional activity. Not involved in RXRA ubiquitination by UBE2E2.
    • Tissue specificity

      Ubiquitous. In fetal tissues, highest expression in brain, thymus and liver. In adult tissues, highest levels in brain and testis, lowest levels in peripheral blood cells.
    • Pathway

      Protein modification; protein ubiquitination.
    • Sequence similarities

      Belongs to the RNF8 family.
      Contains 1 FHA domain.
      Contains 1 RING-type zinc finger.
    • Developmental stage

      Low levels at the G1-S boundary increase in intensity during S phase and until the end of the G2 phase. Abruptly decreases in late mitosis (at protein level). Barely detectable in anaphase.
    • Domain

      The FHA domain specifically recognizes and binds ATM-phosphorylated MDC1 and Thr-4827 phosphorylated HERC2.
    • Post-translational
      modifications

      Autoubiquitinated through 'Lys-48' and 'Lys-63' of ubiquitin. 'Lys-63' polyubiquitination is mediated by UBE2N. 'Lys-29'-type polyubiquitination is also observed, but it doesn't require its own functional RING-type zinc finger.
    • Cellular localization

      Nucleus. Midbody. Following DNA double-strand breaks, recruited to the sites of damage. During prophase, concomitant with nuclear envelope breakdown, localizes throughout the cell, with a dotted pattern. In telophase, again in the nucleus and also with a discrete dotted pattern in the cytoplasm. In late telophase and during cytokinesis, localizes in the midbody of the tubulin bridge joining the daughter cells. Does not seem to be associated with condensed chromosomes at any time during the cell cycle.
    • Information by UniProt
    • Database links

    • Alternative names

      • C3HC4 type zinc finger protein antibody
      • E3 ubiquitin protein ligase RNF8 antibody
      • E3 ubiquitin-protein ligase RNF8 antibody
      • FLJ12013 antibody
      • KIAA0646 antibody
      • Ring finger protein (C3HC4 type) 8 antibody
      • RING finger protein 8 antibody
      • Ring finger protein 8, E3 ubiquitin protein ligase antibody
      • RNF 8 antibody
      • RNF8 antibody
      • RNF8_HUMAN antibody
      • UBC13/UEV interacting ring finger protein antibody
      see all

    Images

    • All lanes : Anti-RNF8 antibody [EPR6312(2)] (ab131221) at 1/1000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : Human fetal thymus lysate
      Lane 3 : Human fetal kidney lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 55 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Wang C  et al. HTLV-1 Tax Functions as a Ubiquitin E3 Ligase for Direct IKK Activation via Synthesis of Mixed-Linkage Polyubiquitin Chains. PLoS Pathog 12:e1005584 (2016). Read more (PubMed: 27082114) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab131221.
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