Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6312(2)] to RNF8
- Suitable for: WB
- Reacts with: Rat, Human
Product nameAnti-RNF8 antibody [EPR6312(2)]
See all RNF8 primary antibodies
DescriptionRabbit monoclonal [EPR6312(2)] to RNF8
Tested applicationsSuitable for: WBmore details
Unsuitable for: ICC,IHC-P or IP
Species reactivityReacts with: Rat, Human
Synthetic peptide within Human RNF8 aa 400-500. The exact sequence is proprietary.
- Human fetal brain, fetal thymus, and fetal kidney lysates.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 5.13 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab131221 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).|
FunctionE3 ubiquitin-protein ligase required for assembly of repair proteins to sites of DNA damage. Catalyzes the 'Lys-63'-linked ubiquitination of histone H2A and H2AX. Following DNA double-strand breaks (DSBs), it is recruited to the sites of damage by ATM-phosphorylated MDC1, mediates the ubiquitination of histones H2A and H2AX, thereby promoting the formation of TP53BP1 and BRCA1 ionizing radiation-induced foci (IRIF). Promotes the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBE2N/UBC13. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation. Enforces the G2/M DNA damage checkpoint. Controls the recruitment of UIMC1-BRCC3 (RAP80-BRCC36) and PAXIP1/PTIP to DNA damage sites following DNA double-strand breaks (DSBs). Ubiquitination of histone H2A requires UBE2N but not MMS2 (UBE2V2). May also ubiquitinate histone H2B. Catalyzes the 'Lys-63'-linked ubiquitination of PCNA. May be required for proper exit from mitosis after spindle checkpoint activation and may regulate cytokinesis. May play a role in the regulation of RXRA-mediated transcriptional activity. Not involved in RXRA ubiquitination by UBE2E2.
Tissue specificityUbiquitous. In fetal tissues, highest expression in brain, thymus and liver. In adult tissues, highest levels in brain and testis, lowest levels in peripheral blood cells.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the RNF8 family.
Contains 1 FHA domain.
Contains 1 RING-type zinc finger.
Developmental stageLow levels at the G1-S boundary increase in intensity during S phase and until the end of the G2 phase. Abruptly decreases in late mitosis (at protein level). Barely detectable in anaphase.
DomainThe FHA domain specifically recognizes and binds ATM-phosphorylated MDC1 and Thr-4827 phosphorylated HERC2.
modificationsAutoubiquitinated through 'Lys-48' and 'Lys-63' of ubiquitin. 'Lys-63' polyubiquitination is mediated by UBE2N. 'Lys-29'-type polyubiquitination is also observed, but it doesn't require its own functional RING-type zinc finger.
Cellular localizationNucleus. Midbody. Following DNA double-strand breaks, recruited to the sites of damage. During prophase, concomitant with nuclear envelope breakdown, localizes throughout the cell, with a dotted pattern. In telophase, again in the nucleus and also with a discrete dotted pattern in the cytoplasm. In late telophase and during cytokinesis, localizes in the midbody of the tubulin bridge joining the daughter cells. Does not seem to be associated with condensed chromosomes at any time during the cell cycle.
- Information by UniProt
- C3HC4 type zinc finger protein antibody
- E3 ubiquitin protein ligase RNF8 antibody
- E3 ubiquitin-protein ligase RNF8 antibody
All lanes : Anti-RNF8 antibody [EPR6312(2)] (ab131221) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal thymus lysate
Lane 3 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 55 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab131221 has been referenced in 1 publication.
- Wang C et al. HTLV-1 Tax Functions as a Ubiquitin E3 Ligase for Direct IKK Activation via Synthesis of Mixed-Linkage Polyubiquitin Chains. PLoS Pathog 12:e1005584 (2016). PubMed: 27082114