Anti-TRIM4 antibody (ab4527)
Key features and details
- Goat polyclonal to TRIM4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TRIM4 antibody
See all TRIM4 primary antibodies -
Description
Goat polyclonal to TRIM4 -
Host species
Goat -
Specificity
This antibody is expected to recognise both human isoforms. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human TRIM4 aa 450 to the C-terminus (C terminal). (NP_149082.1)
Database link: NP_148977.1 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab4527 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 55-58 kDa (predicted molecular weight: 55-60 kDa).
A 1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 55-58 kDa (predicted molecular weight: 55-60 kDa). A 1 hour primary incubation is recommended for this product. |
Target
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Sequence similarities
Belongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger. - Information by UniProt
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Database links
- Entrez Gene: 89122 Human
- SwissProt: Q9C037 Human
- Unigene: 50749 Human
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Alternative names
- RING finger protein 87 antibody
- RNF 87 antibody
- RNF87 antibody
see all
Images
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ab4527 staining (1ug/ml) of HepG2 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot using chemiluminescence.
Datasheets and documents
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Datasheet download
References (1)
ab4527 has been referenced in 1 publication.
- Feyeux M et al. Early transcriptional changes linked to naturally occurring Huntington's disease mutations in neural derivatives of human embryonic stem cells. Hum Mol Genet 21:3883-95 (2012). PubMed: 22678061