Product nameAnti-RNF90 antibody - N-terminal
See all RNF90 primary antibodies
DescriptionRabbit polyclonal to RNF90 - N-terminal
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human RNF90 aa 74-103 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: Q9C029-4
- NCI-H292 and HepG2 cell line lysate; Human skeletal muscle tissue
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab170538 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/500. Predicted molecular weight: 57 kDa.|
|IHC-P||1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Tissue specificitySkeletal muscle and placenta, at lower levels in heart, brain and pancreas. Isoform 1 is widely expressed with high level in testis, kidney and heart.
Sequence similaritiesBelongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger.
DomainThe B30.2 domain mediates interaction with GYG. The coiled-coil region mediates homodimerization and heterodimerization.
- Information by UniProt
- Glycogenin-interacting protein antibody
- GNIP antibody
- RING finger protein 90 antibody
All lanes : Anti-RNF90 antibody - N-terminal (ab170538) at 1/100 dilution
Lane 1 : NCI-H292 cell line lysate
Lane 2 : HepG2 cell line lysate
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 57 kDa
Immunohistochemical analysis of formalin fixed, paraffin embedded Human skeletal muscle tissue labeling RNF90 with ab170538 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB detection.
ab170538 has not yet been referenced specifically in any publications.