Anti-AP-B antibody (ab220088)
Key features and details
- Rabbit polyclonal to AP-B
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-AP-B antibody
See all AP-B primary antibodies -
Description
Rabbit polyclonal to AP-B -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human AP-B aa 500-600.
Database link: Q9H4A4 -
Positive control
- Human gallbladder tissue; RT-4 and U-251 MG cell lysates; Human liver and tonsil lysates; U-2 OS cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab220088 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 73 kDa.
|
|
IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
Notes |
---|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 73 kDa. |
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
Target
-
Function
Exopeptidase which selectively removes arginine and/or lysine residues from the N-terminus of several peptide substrates including Arg(0)-Leu-enkephalin, Arg(0)-Met-enkephalin and Arg(-1)-Lys(0)-somatostatin-14. Can hydrolyze leukotriene A4 (LTA-4) into leukotriene B4 (LTB-4). -
Sequence similarities
Belongs to the peptidase M1 family. -
Cellular localization
Secreted. - Information by UniProt
-
Database links
- Entrez Gene: 6051 Human
- Entrez Gene: 215615 Mouse
- Entrez Gene: 81761 Rat
- Omim: 602675 Human
- SwissProt: Q9H4A4 Human
- SwissProt: Q8VCT3 Mouse
- SwissProt: O09175 Rat
- Unigene: 497391 Human
see all -
Alternative names
- Aminopeptidase B antibody
- AMPB_HUMAN antibody
- Ap B antibody
see all
Images
-
All lanes : Anti-AP-B antibody (ab220088) at 1/100 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Predicted band size: 73 kDa -
Immunohistochemical analysis of paraffin-embedded human gallbladder tissue labeling AP-B with ab220088 at 1/500 dilution.
-
Immunofluorecent analysis of PFA-fixed, Triton X-100 permeabilized U-2 OS cells labeling AP-B with ab220088 at 4 µg/ml (green).
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab220088 has not yet been referenced specifically in any publications.