Ro 31-8220 mesylate (Bisindolylmaleimide IX), Protein kinase inhibitor (ab120374)
- Datasheet
- References (1)
Overview
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Product name
Ro 31-8220 mesylate (Bisindolylmaleimide IX), Protein kinase inhibitor -
Description
Protein kinase inhibitor -
Alternative names
- Bisindolylmaleimide IX
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Biological description
Widely used as a cell-permeable, reversible, competitive protein kinase C inhibitor. Also potently inhibits wide range of kinases inlcuding RSK1, RSK2, PRK2, PKCα, PKD1, MSK1, GSK3β, CDK2-cyclin A and PIM3, as well as PKCα.
Also available in simple stock solutions (ab146723) - add 1 ml of water to get an exact, ready-to-use concentration. -
Purity
> 98% -
CAS Number
125314-64-9 -
Chemical structure
Properties
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Chemical name
3-[1-[3-(Amidinothio)propyl]-3-indolyl]-4-(1-methyl-3-indolyl)-1H-pyrrole-2,5-dione methanesulfonate -
Molecular weight
553.65 -
Molecular formula
C25H23N5O2S.CH3SO3H -
PubChem identifier
11628205 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in water to 100 mM, DMSO to 100 mM and in ethanol to 5 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
CS(=O)(=O)O.Cn2cc(c1ccccc12)C=5C(=O)NC(=O)C=5c4cn(CCCSC(=N)N)c3ccccc34 -
Source
Synthetic
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Research areas
Images
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Functional Studies - Ro 31-8220 mesylate (Bisindolylmaleimide IX), Protein kinase inhibitor (ab120374)1.5 x 10e7 THP-1 cells were incubated with 100 nM GF 109203X (ab144264) or Ro 31-8220 mesylate (ab120374) for 30 minutes prior to activation with 10 µg x mL-1 PMA (Sigma) for 4 hours. Control cells were left without inhibitors or PMA. Cells were lysed in 1 mL of lysis buffer, and 30 µL were tested for PKC activity (duplicates; +/- SD).
References
This product has been referenced in:
- Bain J et al. The selectivity of protein kinase inhibitors: a further update. Biochem J 408:297-315 (2007). Read more (PubMed: 17850214) »
- Davis PD et al. Inhibitors of protein kinase C. 2. Substituted bisindolylmaleimides with improved potency and selectivity. J Med Chem 35:994-1001 (1992). Read more (PubMed: 1552513) »
- Dieter P & Fitzke E RO 31-8220 and RO 31-7549 show improved selectivity for protein kinase C over staurosporine in macrophages. Biochem Biophys Res Commun 181:396-401 (1991). Read more (PubMed: 1958208) »
