For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    ro-31-8220-mesylate-bisindolylmaleimide-ix-protein-kinase-inhibitor-ab120374.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Neuroscience Neurotransmission Intracellular Signaling Kinases
Share by email

Ro 31-8220 mesylate (Bisindolylmaleimide IX), Protein kinase inhibitor (ab120374)

  • Datasheet
  • SDS
Submit a review Submit a question References (1)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Functional Studies - Ro 31-8220 mesylate (Bisindolylmaleimide IX), Protein kinase inhibitor (ab120374)

    Key features and details

    • Protein kinase inhibitor
    • CAS Number: 125314-64-9
    • Purity: > 98%
    • Soluble in DMSO and organic solvents. Solubility in these solvents is approximately 20 mg/mL.

    • Form / State: Solid
    • Source: Synthetic

    Overview

    • Product name

      Ro 31-8220 mesylate (Bisindolylmaleimide IX), Protein kinase inhibitor
    • Description

      Protein kinase inhibitor
    • Alternative names

      • Bisindolylmaleimide IX
    • Biological description

      Widely used as a cell-permeable, reversible, competitive protein kinase C inhibitor. Also potently inhibits wide range of kinases inlcuding RSK1, RSK2, PRK2, PKCα, PKD1, MSK1, GSK3β, CDK2-cyclin A and PIM3, as well as PKCα.

      Also available in simple stock solutions (ab146723) - add 1 ml of water to get an exact, ready-to-use concentration.
    • Purity

      > 98%
    • CAS Number

      125314-64-9
    • Chemical structure

      Chemical Structure

    Properties

    • Chemical name

      3-[1-[3-(Amidinothio)propyl]-3-indolyl]-4-(1-methyl-3-indolyl)-1H-pyrrole-2,5-dione methanesulfonate

    • Molecular weight

      553.65
    • Molecular formula

      C25H23N5O2S.CH3SO3H
    • PubChem identifier

      11628205
    • Storage instructions

      Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
    • Solubility overview

      Soluble in DMSO and organic solvents. Solubility in these solvents is approximately 20 mg/mL.

    • Handling

      Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

      Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

    • SMILES

      CS(=O)(=O)O.Cn2cc(c1ccccc12)C=5C(=O)NC(=O)C=5c4cn(CCCSC(=N)N)c3ccccc34
    • Source

      Synthetic

    • Research areas

      • Neuroscience
      • Neurotransmission
      • Intracellular Signaling
      • Kinases
      • Epigenetics and Nuclear Signaling
      • Chromatin Modifying Enzymes
      • Acetylation
      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Microtubules
      • Tubulin
      • Signal Transduction
      • Protein Phosphorylation
      • Ser / Thr Kinases
      • PKC
      • Signal Transduction
      • Protein Phosphorylation
      • Ser / Thr Kinases
      • MAPK Pathway
      • Signal Transduction
      • Protein Phosphorylation
      • Ser / Thr Kinases
      • Other Kinases
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Other factors
      • Stem Cells
      • Signaling Pathways
      • TGF beta
      • Cytoplasmic
      • Epigenetics and Nuclear Signaling
      • Chromatin Modifying Enzymes
      • Acetylation
      • HDACs
      • Class I
      • Biochemicals
      • Product Range
      • Just Add Water
      • Biochemicals
      • Chemical Type
      • Biochemicals
      • Biochemicals
      • Pharmacology
      • Enzymes
      • Kinase
      • General
      • Inhibitors
      • Biochemicals
      • Pharmacology
      • Enzymes
      • Kinase
      • Protein Kinase C
      • Inhibitors

    Images

    • Functional Studies - Ro 31-8220 mesylate (Bisindolylmaleimide IX), Protein kinase inhibitor (ab120374)
      Functional Studies - Ro 31-8220 mesylate (Bisindolylmaleimide IX), Protein kinase inhibitor (ab120374)

      1.5 x 10e7 THP-1 cells were incubated with 100 nM GF 109203X (ab144264) or Ro 31-8220 mesylate (ab120374) for 30 minutes prior to activation with 10 µg x mL-1 PMA (Sigma) for 4 hours. Control cells were left without inhibitors or PMA. Cells were lysed in 1 mL of lysis buffer, and 30 µL were tested for PKC activity (duplicates; +/- SD).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (1)

    Publishing research using ab120374? Please let us know so that we can cite the reference in this datasheet.

    ab120374 has been referenced in 1 publication.

    • Li Y  et al. A novel perspective and approach to intestinal octreotide absorption: sinomenine-mediated reversible tight junction opening and its molecular mechanism. Int J Mol Sci 14:12873-92 (2013). PubMed: 23787475

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab120374.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.