Anti-Robo1 antibody (ab7279)
Key features and details
- Rabbit polyclonal to Robo1
- Suitable for: ELISA, IHC-P, WB, IHC-Fr
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Robo1 antibody
See all Robo1 primary antibodies -
Description
Rabbit polyclonal to Robo1 -
Host species
Rabbit -
Specificity
Some batches may cross-react with Robo2 and Robo3. Please see abreview for more details. -
Tested applications
Suitable for: ELISA, IHC-P, WB, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide:
C-VLGGYERGEDNNE
conjugated to KLH, corresponding to amino acids 1632-1644 of Human Robo1. -
Positive control
- WB: HEK-293, HeLa, MCF-7, A-549, and A-172 lysates. IHC-P: Human brain tissue. IHC-Fr: Mouse lung tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7279 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
1/30000 - 1/60000.
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IHC-P | (3) |
Use a concentration of 2 - 10 µg/ml.
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WB | (4) |
1/500 - 1/3000. Detects a band of approximately 181 kDa.
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IHC-Fr | (4) |
Use a concentration of 2 - 10 µg/ml.
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Notes |
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ELISA
1/30000 - 1/60000. |
IHC-P
Use a concentration of 2 - 10 µg/ml. |
WB
1/500 - 1/3000. Detects a band of approximately 181 kDa. |
IHC-Fr
Use a concentration of 2 - 10 µg/ml. |
Target
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Function
Receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. May be required for lung development. -
Tissue specificity
Widely expressed, with exception of kidney. -
Sequence similarities
Belongs to the immunoglobulin superfamily. ROBO family.
Contains 3 fibronectin type-III domains.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsUbiquitinated. May be deubiquitinated by USP33. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6091 Human
- Entrez Gene: 19876 Mouse
- Omim: 602430 Human
- SwissProt: Q9Y6N7 Human
- SwissProt: O89026 Mouse
- Unigene: 13640 Human
- Unigene: 310772 Mouse
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Alternative names
- Deleted in U twenty twenty antibody
- DUTT 1 antibody
- DUTT1 antibody
see all
Images
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Anti-Robo1 antibody (ab7279) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
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This image shows staining of human brain tissue.
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Immunohistochemistry analysis of mouse lung tissue sections (adult, frozen 100 µm wholemount sections) labelling Robo1 with ab7279 at 1/50 dilution. The tissue was paraformaldehyde fixed and permeabilized with triton x-100 before incubation with the antibody for 16 hours at 4°C.
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Anti-Robo1 antibody (ab7279) at 1/1000 dilution + HEK 293T cells at 25 µg
Developed using the ECL technique.
Performed under reducing conditions.
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All lanes : Anti-Robo1 antibody (ab7279) at 1 µg/ml
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 5 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 6 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 7 : LNCaP (human prostate cancer cell line) whole cell lysate
Lane 8 : MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate
Lane 9 : Ramos (human Burkitt's lymphoma cell line) whole cell lysate
Lane 10 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 11 : A-172 (human brain glioblastoma) whole cell lysate
Lane 12 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 35 µg per lane.
Secondary
All lanes : Peroxidase Rabbit secondary antibody for 60 minutes at room temperature at 1/30000 dilution
Observed band size: 181 kDa why is the actual band size different from the predicted?Primary antibody incubated overnight at 4C.
Blocking Buffer: 1% Casein-TTBS for 30 min at room temperature.
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ELISA results of purified ab7279 tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1µg of conjugate. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated secondary and TMB ELISA Peroxidase Substrate.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (50)
ab7279 has been referenced in 50 publications.
- Yin T & Zhao H miR-152-3p impedes the malignant phenotypes of hepatocellular carcinoma by repressing roundabout guidance receptor 1. Cell Mol Biol Lett 27:22 (2022). PubMed: 35236289
- Feng XF et al. Magnetic Resonance Imaging Investigation of Neuroplasticity After Ischemic Stroke in Tetramethylpyrazine-Treated Rats. Front Pharmacol 13:851746 (2022). PubMed: 35559236
- Bianchi G et al. ROBO1 Promotes Homing, Dissemination, and Survival of Multiple Myeloma within the Bone Marrow Microenvironment. Blood Cancer Discov 2:338-353 (2021). PubMed: 34268498
- Kim SH et al. ROBO1 protein expression is independently associated with biochemical recurrence in prostate cancer patients who underwent radical prostatectomy in Asian patients. Gland Surg 10:2956-2965 (2021). PubMed: 34804883
- Jian Y & Fan Q Long non-coding RNA SNHG7 facilitates pancreatic cancer progression by regulating the miR-146b-5p/Robo1 axis. Exp Ther Med 21:398 (2021). PubMed: 33680120