Product nameAnti-Robo1 antibody
See all Robo1 primary antibodies
DescriptionRabbit polyclonal to Robo1
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human Robo1 aa 1600 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following tissue lysates: Human brain; Mouse brain.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab85312 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 190 kDa (predicted molecular weight: 180 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionReceptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. May be required for lung development.
Tissue specificityWidely expressed, with exception of kidney.
Sequence similaritiesBelongs to the immunoglobulin superfamily. ROBO family.
Contains 3 fibronectin type-III domains.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
modificationsUbiquitinated. May be deubiquitinated by USP33.
- Information by UniProt
- Deleted in U twenty twenty antibody
- DUTT 1 antibody
- DUTT1 antibody
All lanes : Anti-Robo1 antibody (ab85312) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 180 kDa
Observed band size: 190 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
Human Roundabout homolog 1 precursor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
ICC/IF image of ab85312 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85312, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Zhan Y et al. An MRI Study of Neurovascular Restorative After Combination Treatment With Xiaoshuan Enteric-Coated Capsule and Enriched Environment in Rats After Stroke. Front Neurosci 13:701 (2019). Read more (PubMed: 31354412) »
- Smeda M et al. Nitric oxide deficiency and endothelial-mesenchymal transition of pulmonary endothelium in the progression of 4T1 metastatic breast cancer in mice. Breast Cancer Res 20:86 (2018). Read more (PubMed: 30075800) »