IHC on 20µm frozen section of spinal cord. Embryos were fixed by immersion in 4% paraformaldehyde in 0.12 M phosphate buffer, pH 7.4 (PFA) and then cryoprotected in 10% sucrose. Cryostat sections were blocked in 0.2% gelatin in PBS containing 0.25% Triton X-100 and incubated overnight at room temperature in the same buffer.
We appreciate the customer’s feedback. We had not tested the antibody in IHC-Fr previously and so we did not know how well the antibody would work in this application.
Dr. Alain Chedotal
Submitted Jun 30 2012
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