Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Robo1 antibody

Poor
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (spinal cord)
Specification
spinal cord
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
Yes - Triton
Blocking step
Gelatine as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 22°C

Other product details

Dilution
1/100
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 22°C

Secondary antibody

Name
Non-Abcam antibody was used: CY3 conjugated donkey anti-rabbit antibody
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/300

Additional data

Additional Notes
IHC on 20µm frozen section of spinal cord.
Embryos were fixed by immersion in 4% paraformaldehyde in 0.12 M phosphate buffer, pH 7.4 (PFA) and then cryoprotected in 10% sucrose. Cryostat sections were blocked in 0.2% gelatin in PBS containing 0.25% Triton X-100 and incubated overnight at room temperature in the same buffer.
Abcam response
We appreciate the customer’s feedback. We had not tested the antibody in IHC-Fr previously and so we did not know how well the antibody would work in this application.

Dr. Alain Chedotal

Verified customer

Submitted Jun 30 2012

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