Anti-Robo2 antibody (ab244331)
Key features and details
- Rabbit polyclonal to Robo2
- Suitable for: ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Robo2 antibody
See all Robo2 primary antibodies -
Description
Rabbit polyclonal to Robo2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human Robo2 aa 900-1100.
Database link: Q9HCK4 -
Positive control
- IHC-P: Mouse embryo E14, human cerebral cortex and placenta tissue; ICC/IF: SH-SY5Y cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab244331 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
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IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Receptor for SLIT2, and probably SLIT1, which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. -
Involvement in disease
Defects in ROBO2 are the cause of vesicoureteral reflux type 2 (VUR2) [MIM:610878]. VUR is a complex, genetically heterogeneous developmental disorder characterized by the retrograde flow of urine from the bladder into the ureter and is associated with reflux nephropathy, the cause of 15% of end-stage renal disease in children and young adults.
Note=A chromosomal aberration involving ROBO2 is a cause of multiple congenital abnormalities, including severe bilateral VUR with ureterovesical junction defects. Translocation t(Y;3)(p11;p12) with PCDH11Y. This translocation disrupts ROBO2 and produces dominant-negative ROBO2 proteins that abrogate SLIT-ROBO signaling in vitro. -
Sequence similarities
Belongs to the immunoglobulin superfamily. ROBO family.
Contains 3 fibronectin type-III domains.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6092 Human
- Entrez Gene: 268902 Mouse
- Omim: 602431 Human
- SwissProt: Q9HCK4 Human
- SwissProt: Q7TPD3 Mouse
- Unigene: 13305 Human
- Unigene: 171736 Mouse
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Alternative names
- lea antibody
- Robo 2 antibody
- Robo2 antibody
see all
Images
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Immunohistochemical analysis of mouse embryo E14 tissue labeling Robo2 in developing olfatory bulb and olfactory nerve with ab244331 at 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent analysis of SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells labeling Robo2 (green) in the nucleus with ab244331 at 2 µg/ml.
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Immunohistochemical analysis of human cerebral cortex tissue labeling Robo2 in neuronal processes in neuropil with ab244331 at 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of human placenta tissue labeling Robo2 in the cytoplasm of trophoblastic cels with ab244331 at 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of human liver tissue labeling Robo2 (very weak positivity) with ab244331 at 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of human kidney tissue labeling Robo2 with ab244331 at 1/20 dilution. Negative control.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab244331 has not yet been referenced specifically in any publications.