Product nameAnti-Robo2 antibody
See all Robo2 primary antibodies
DescriptionRabbit polyclonal to Robo2
Specificityab75014 has shown to not cross react with human Robo1 or Robo3 protein.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Mouse Robo2 aa 1250-1350 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following tissue lysates: Rat Liver Mouse Liver
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab75014 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 171 kDa (predicted molecular weight: 161 kDa).|
FunctionReceptor for SLIT2, and probably SLIT1, which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development.
Involvement in diseaseDefects in ROBO2 are the cause of vesicoureteral reflux type 2 (VUR2) [MIM:610878]. VUR is a complex, genetically heterogeneous developmental disorder characterized by the retrograde flow of urine from the bladder into the ureter and is associated with reflux nephropathy, the cause of 15% of end-stage renal disease in children and young adults.
Note=A chromosomal aberration involving ROBO2 is a cause of multiple congenital abnormalities, including severe bilateral VUR with ureterovesical junction defects. Translocation t(Y;3)(p11;p12) with PCDH11Y. This translocation disrupts ROBO2 and produces dominant-negative ROBO2 proteins that abrogate SLIT-ROBO signaling in vitro.
Sequence similaritiesBelongs to the immunoglobulin superfamily. ROBO family.
Contains 3 fibronectin type-III domains.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
- Information by UniProt
- lea antibody
- Robo 2 antibody
- Robo2 antibody
Lanes 1-3 : Anti-Robo2 antibody (ab75014) at 2 µg/ml
Lanes 4-6 : Mouse Monoclonal to V5 at 1/1000 dilution
Lanes 1 & 4 : HEK293 cells expressing Robo1
Lanes 2 & 5 : HEK293 cells expressing Robo2-V5
Lanes 3 & 6 : HEK293 cells expressing Robo3
Lysates/proteins at 10 µg per lane.
Lanes 1-3 : Anti-Rabbit HRP at 1/20000 dilution
Lanes 4-6 : Anti-Mouse HRP
Predicted band size: 161 kDa
All lanes : Anti-Robo2 antibody (ab75014) at 1 µg/ml
Lane 1 : Liver (Rat) Tissue Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 161 kDa
Exposure time: 20 minutes
This product has been referenced in:
- Pinho AV et al. ROBO2 is a stroma suppressor gene in the pancreas and acts via TGF-ß signalling. Nat Commun 9:5083 (2018). Read more (PubMed: 30504844) »
- Xu R et al. Targeting skeletal endothelium to ameliorate bone loss. Nat Med 24:823-833 (2018). Read more (PubMed: 29785024) »