Key features and details
- Goat polyclonal to Robo3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Robo3 antibody
See all Robo3 primary antibodies
DescriptionGoat polyclonal to Robo3
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Horse, Dog, Zebrafish, Rhesus monkey
- Human Brain (Cerebellum and Hippocampus) lysates.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab77261 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
Our Abpromise guarantee covers the use of ab77261 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 148 kDa (predicted molecular weight: 148 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionThought to be involved during neural development in axonal navigation at the ventral midline of the neural tube. In spinal chord development plays a role in guiding commissural axons probably by preventing premature sensitivity to Slit proteins thus inhibiting Slit signaling through ROBO1 (By similarity). Required for hindbrain axon midline crossing.
Involvement in diseaseDefects in ROBO3 are a cause of familial horizontal gaze palsy with progressive scoliosis (HGPPS) [MIM:607313]. Patients show a medulla where motor and sensory projections appear uncrossed.
Sequence similaritiesBelongs to the immunoglobulin superfamily. ROBO family.
Contains 3 fibronectin type-III domains.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
- Information by UniProt
- FLJ21044 antibody
- HGPPS antibody
- HGPS antibody
ab77261 has not yet been referenced specifically in any publications.