Key features and details
- Rabbit monoclonal [EPR23426-145] to Robo4 - BSA and Azide free
- Suitable for: Flow Cyt, WB
- Reacts with: Human
Product nameAnti-Robo4 antibody [EPR23426-145] - BSA and Azide free
See all Robo4 primary antibodies
DescriptionRabbit monoclonal [EPR23426-145] to Robo4 - BSA and Azide free
Tested applicationsSuitable for: Flow Cyt, WBmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Human
Recombinant fragment within Human Robo4 aa 1-500. The exact sequence is proprietary.
Database link: Q8WZ75
- WB: HUVEC whole cell lysate. Flow Cyt: HUVEC cells.
ab273519 is the carrier-free version of ab272734. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab273519 in the following tested applications.
|Flow Cyt||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 107 kDa).|
- Information by UniProt
- ECSM4 antibody
- FLJ20798 antibody
- Magic roundabout antibody
Flow cytometric analysis of HeLa (Human cervix adenocarcinoma epithelial cell) (Left panel) / HUVEC (Human umbilical vein endothelial cell) (Right panel) cells labelling Robo4 with ab272734 at 1/50 dilution (1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody.
Negative control: HeLa (PMID: 11076864).
Gated on viable cells.
Brighter secondary antibody like Alexa Fluor® 647 is required to get desirable staining.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272734).
ab273519 has not yet been referenced specifically in any publications.