Anti-ROCK1 antibody (ab97592)
Key features and details
- Rabbit polyclonal to ROCK1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-ROCK1 antibody
See all ROCK1 primary antibodies -
Description
Rabbit polyclonal to ROCK1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Rabbit, Cow, Zebrafish -
Immunogen
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 107 and 381 of human ROCK1 (Q13464)
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Positive control
- 293T , A431 , HeLa , HepG2 , NIH-3T3 , C2Cl2 , rat brain
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97592 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 158 kDa.
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IHC-P |
1/100 - 1/1000.
Antigen Retrieval: EDTA based, pH 8.0 buffer, 15min |
Notes |
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ICC/IF
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 158 kDa. |
IHC-P
1/100 - 1/1000. Antigen Retrieval: EDTA based, pH 8.0 buffer, 15min |
Target
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Function
Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. -
Tissue specificity
Detected in blood platelets. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 REM (Hr1) repeat. -
Domain
The C-terminal auto-inhibitory domain interferes with kinase activity. RHOA binding leads to a conformation change and activation of the kinase. Truncated ROCK1 is constitutively activated. -
Post-translational
modificationsAutophosphorylated on serine and threonine residues.
Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing. -
Cellular localization
Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Golgi apparatus membrane. Cell projection, bleb. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, ruffle. Associated with the mother centriole and an intercentriolar linker. Colocalizes with ITGB1BP1 and ITGB1 at the cell membrane predominantly in lamellipodia and membrane ruffles, but also in retraction fibers. Localizes at the cell membrane in an ITGB1BP1-dependent manner (By similarity). A small proportion is associated with Golgi membranes. - Information by UniProt
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Database links
- Entrez Gene: 785911 Cow
- Entrez Gene: 6093 Human
- Entrez Gene: 100009220 Rabbit
- Entrez Gene: 81762 Rat
- Omim: 601702 Human
- SwissProt: Q8MIT6 Cow
- SwissProt: Q13464 Human
- SwissProt: O77819 Rabbit
see all -
Alternative names
- coiled-coil-containing protein kinase 1 antibody
- coiled-coil-containing protein kinase I antibody
- MGC131603 antibody
see all
Images
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All lanes : Anti-ROCK1 antibody (ab97592) at 1/1000 dilution
Lane 1 : Wild-type HeLa whole cell lysate
Lane 2 : ROCK1 knockout HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 158 kDa5% SDS-PAGE
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Immunofluorescence staining of paraformaldehyde-fixed (4% paraformaldehyde, 15 minutes at RT) HeLa cells stained for ROCK1 with ab97592 at 1:500 dilution. Red: alpha Tubulin, a cytoskeleton marker, stained by an alpha Tubulin antibody diluted at 1:500.
Blue: Hoechst 33342 staining. -
Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue labelling ROCK1 with ab97592 antibody at 1/500 dilution.
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All lanes :
Lane 1 : 293T whole cell extracts
Lane 2 : A431 whole cell extracts
Lane 3 : HeLa whole cell extracts
Lane 4 : HepG2 whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 158 kDa5% SDS-PAGE
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Anti-ROCK1 antibody (ab97592) at 1/500 dilution + Rat brain lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 158 kDa -
ab97592, at a 1/500 dilution, staining human ROCK1 in DLD1 xenograft, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (18)
ab97592 has been referenced in 18 publications.
- Song S et al. LncRNA BCYRN1 promotes cell migration and invasion of non-small cell lung cancer via the miR-30b-3p/ROCK1 axis. Neoplasma 69:583-593 (2022). PubMed: 35225646
- Sharifi M et al. Necroptosis and RhoA/ROCK pathways: molecular targets of Nesfatin-1 in cardioprotection against myocardial ischemia/reperfusion injury in a rat model. Mol Biol Rep 48:2507-2518 (2021). PubMed: 33755849
- Diao H et al. miR-135a-5p inhibits tumor invasion by targeting ANGPT2 in gallbladder cancer. Mol Med Rep 24:N/A (2021). PubMed: 34036386
- Zhou W et al. miR-217 alleviates high-glucose-induced vascular smooth muscle cell dysfunction via regulating ROCK1. J Biochem Mol Toxicol 35:e22668 (2021). PubMed: 33283391
- Feng N et al. Circ_PIP5K1A regulates cisplatin resistance and malignant progression in non-small cell lung cancer cells and xenograft murine model via depending on miR-493-5p/ROCK1 axis. Respir Res 22:248 (2021). PubMed: 34537072