• Product name
  • Description
    Rabbit polyclonal to ROCK1
  • Host species
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Zebrafish
  • Immunogen

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 107 and 381 of human ROCK1 (Q13464)

  • Positive control
    • 293T, A431, H1299, HeLa, HepG2, Molt-4 or Raji cell lysate DLD1 xenograft HeLa cells



Our Abpromise guarantee covers the use of ab97592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/200.
WB 1/500 - 1/3000. Predicted molecular weight: 158 kDa.
IHC-P 1/100 - 1/500.


  • Function
    Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization.
  • Tissue specificity
    Detected in blood platelets.
  • Sequence similarities
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 PH domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
    Contains 1 REM (Hr1) repeat.
  • Domain
    The C-terminal auto-inhibitory domain interferes with kinase activity. RHOA binding leads to a conformation change and activation of the kinase. Truncated ROCK1 is constitutively activated.
  • Post-translational
    Autophosphorylated on serine and threonine residues.
    Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing.
  • Cellular localization
    Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Golgi apparatus membrane. Cell projection, bleb. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, ruffle. Associated with the mother centriole and an intercentriolar linker. Colocalizes with ITGB1BP1 and ITGB1 at the cell membrane predominantly in lamellipodia and membrane ruffles, but also in retraction fibers. Localizes at the cell membrane in an ITGB1BP1-dependent manner (By similarity). A small proportion is associated with Golgi membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • coiled-coil-containing protein kinase 1 antibody
    • coiled-coil-containing protein kinase I antibody
    • MGC131603 antibody
    • MGC43611 antibody
    • p160 Rhoassociated coiled coil-forming protein kinase antibody
    • p160 ROCK-1 antibody
    • p160 ROCK1 antibody
    • p160ROCK antibody
    • PRO0435 antibody
    • Renal carcinoma antigen NY REN 35 antibody
    • Renal carcinoma antigen NY-REN-35 antibody
    • Rho associated coiled coil containing protein kinase 1 antibody
    • Rho associated protein kinase 1 antibody
    • Rho kinase antibody
    • Rho-alpha kinase antibody
    • Rho-associated antibody
    • Rho-associated protein kinase 1 antibody
    • ROCK I antibody
    • ROCK-I antibody
    • ROCK1 antibody
    • ROCK1_HUMAN antibody
    • Rok antibody
    • rokalpha antibody
    see all


  • All lanes : Anti-ROCK1 antibody (ab97592) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate
    Lane 3 : MOLT-4 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 158 kDa

    Gel conc 5% (SDS PAGE)
  • ab97592, at a 1/500 dilution, staining human ROCK1 in DLD1 xenograft, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
  • Immunofluorescence analysis of methanol-fixed HeLa, using ab97592 at a 1/200 dilution (top image) and costained with a fluorescent stain (bottom image).


This product has been referenced in:
  • Hou L  et al. Integrin CD11b mediates a-synuclein-induced activation of NADPH oxidase through a Rho-dependent pathway. Redox Biol 14:600-608 (2018). Read more (PubMed: 29154191) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for confirming these details and for your cooperation.

As requested, I have issued a free of charge replacement of alternative ab134181 Anti-ROCK1 antibody [EPR638Y]with the order number #####.

To check the status of the...

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Thank you once again for your message. Thank you also for your patience waiting for a response, I wanted to ensure I had time to review the whole case again fully before contacting you as it has been a while since we last corresponded.

I am ...

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Thank you for your message. I am sorry to hear that the alternative free of charge replacement has also not worked.

It is regrettable both the antibodies have not provided satisfactory results. I would like to reassure you that ab97592 is te...

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Thank you for confirming these details and for your cooperation.

I am sorry that ab45171 has not been working for you and for the inconvenience this has caused. As requested, I have issued an alternative free of charge replacement of 97592 w...

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Thank you for your reply, which has been forwarded to me by my colleague Thomas as I was originally dealing with this case.

I am sorry to hear you have had difficulty with the replacementvials.I am concerned that you have used severalvials o...

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Thank you for contacting us. Ab97592 is 89% homologous with ROCK2 and is therefore likely to cross-react. Ab56661 is 59% homologous with ROCK1 and may or may not cross-react. Unfortunately, we have not tested these antibodies for cross-react...

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