Recombinant Anti-ROCK1 antibody [EPR638Y] (ab134181)
- Datasheet
- References (10)
- Protocols
Overview
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Product nameAnti-ROCK1 antibody [EPR638Y]
See all ROCK1 primary antibodies -
DescriptionRabbit monoclonal [EPR638Y] to ROCK1
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, IHC-Fr, WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human ROCK1 aa 1300-1400 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: HeLa cell lysate, HEK293 cell lysate IHC: Human lymphoma tissue.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR638Y
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Corresponding Antibody
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab134181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/50 - 1/100. | |
| IHC-Fr | Use at an assay dependent concentration. PubMed: 24465504 | |
| WB | 1/500 - 1/1000. Detects a band of approximately 160 kDa (predicted molecular weight: 158 kDa). | |
| IP | 1/60. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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FunctionProtein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization.
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Tissue specificityDetected in blood platelets.
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Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 REM (Hr1) repeat. -
DomainThe C-terminal auto-inhibitory domain interferes with kinase activity. RHOA binding leads to a conformation change and activation of the kinase. Truncated ROCK1 is constitutively activated.
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Post-translational
modificationsAutophosphorylated on serine and threonine residues.
Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing. -
Cellular localizationCytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Golgi apparatus membrane. Cell projection, bleb. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, ruffle. Associated with the mother centriole and an intercentriolar linker. Colocalizes with ITGB1BP1 and ITGB1 at the cell membrane predominantly in lamellipodia and membrane ruffles, but also in retraction fibers. Localizes at the cell membrane in an ITGB1BP1-dependent manner (By similarity). A small proportion is associated with Golgi membranes.
- Information by UniProt
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Database links
- Entrez Gene: 6093 Human
- Entrez Gene: 19877 Mouse
- Entrez Gene: 81762 Rat
- Omim: 601702 Human
- SwissProt: Q13464 Human
- SwissProt: P70335 Mouse
- SwissProt: Q63644 Rat
- Unigene: 306307 Human
see all -
Alternative names
- coiled-coil-containing protein kinase 1 antibody
- coiled-coil-containing protein kinase I antibody
- MGC131603 antibody
see all
Images
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![Western blot - Anti-ROCK1 antibody [EPR638Y] (ab134181)](https://www.abcam.com/ps/products/134/ab134181/Images/ab134181-283473-ab134181GDBKOWB.jpg)
Western blot - Anti-ROCK1 antibody [EPR638Y] (ab134181)Lane 1: Wild type HAP1 whole cell lysate (40 µg)
Lane 2: ROCK1 knockout HAP1 whole cell lysate (40 µg)
Lane 3: HEK293 whole cell lysate (20 µg)
Lane 4: HeLa whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab134181 observed at 165 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab134181 was shown to specifically react with ROCK1 when ROCK1 knockout samples were used. Wild-type and ROCK1 knockout samples were subjected to SDS-PAGE. Ab134181 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 500 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-ROCK1 antibody [EPR638Y] (ab134181)](https://www.abcam.com/ps/products/134/ab134181/Images/ROCK1-Primary-antibodies-ab134181-5.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ROCK1 antibody [EPR638Y] (ab134181)Image courtesy of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canadaab134181 (1/200) staining ROCK1 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview. -
![Western blot - Anti-ROCK1 antibody [EPR638Y] (ab134181)](https://www.abcam.com/ps/products/134/ab134181/Images/ROCK1-Primary-antibodies-ab134181-1.jpg)
Western blot - Anti-ROCK1 antibody [EPR638Y] (ab134181)Anti-ROCK1 antibody [EPR638Y] (ab134181) at 1/500 dilution + HeLa cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 158 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK1 antibody [EPR638Y] (ab134181)](https://www.abcam.com/ps/products/134/ab134181/Images/ROCK1-Primary-antibodies-ab134181-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK1 antibody [EPR638Y] (ab134181)Immunohistochemical analysis of paraffin embedded Human lymphoma tissue labelling ROCK1 with ab134181 at a dilution of 1/50.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Tang H et al. Loss of CLOCK under high glucose upregulates ROCK1-mediated endothelial to mesenchymal transition and aggravates plaque vulnerability. Atherosclerosis 275:58-67 (2018). WB ; Human . Read more (PubMed: 29860109) »
- Stuelten CH et al. Lysophosphatidic acid regulates the motility of MCF10CA1a breast cancer cell sheets via two opposing signaling pathways. Cell Signal 45:1-11 (2018). Read more (PubMed: 29337044) »
