RecombinantRabMAb

Recombinant Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)

Reviews (7)Q&A (11)References (81)
Western blot - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)
  • Immunocytochemistry/ Immunofluorescence - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)
  • Flow Cytometry - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)
  • Western blot - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)
  • Immunoprecipitation - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)
  • Western blot - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)
  • Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP786Y] to ROCK2 + ROCK1
  • Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ROCK2 + ROCK1 antibody [EP786Y]

  • Description

    Rabbit monoclonal [EP786Y] to ROCK2 + ROCK1

  • Host species

    Rabbit

  • Specificity

    This antibody recognizes both the cleaved C-terminus of ROCK 1 (30 kDa) and full length protein (158 kDa). The immunogen used for this product shares 83% homology with ROCK2 and has been shown to bind recombinant human ROCK2, please see western blot images below.

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow

  • Immunogen

    Synthetic peptide within Human ROCK1 aa 1100-1200 (C terminal). The exact sequence is proprietary.
    Database link: Q13464

  • Positive control

    • WB: Untreated, Calyculin A treated and Camptothecin treated HeLa whole cell lysate (ab150035). Jurkat, Ramos, PC-12 and RAW264.7 cell lysates. IHC-P: Human adenocarcinoma of the colon and thyroid gland carcinoma tissues. ICC/IF: HeLa cells. Flow Cyt: HeLa cells. IP: HeLa whole cell lysate (ab150035).

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab45171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 158 kDa.

For unpurified use at 1/500.
IHC-P 1/50 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
ICC/IF 1/50 - 1/500.

 
IP 1/40 - 1/50.

 
Flow Cyt 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

Images

  • Western blot - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)
    Western blot - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)
    All lanes : Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171) at 1/5000 dilution (purified)

    Lane 1 : HeLa cell lysate - treated with Calyculin A
    Lane 2 : HeLa cell lysate - treated with Camptothecin
    Lane 3 : HeLa cell lysate
    Lane 4 : Jurkat cell lysate
    Lane 5 : Ramos cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 158 kDa
    Observed band size: 158 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human adenocarcinoma of the colon tissue labelling ROCK2 + ROCK1 with purified ab45171 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunocytochemistry/ Immunofluorescence - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)
    Immunocytochemistry/ Immunofluorescence - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling ROCK2 + ROCK1 with purified ab45171 at 1/50. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150078, an Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

    Control: primary antibody (1/50) and secondary antibody, ab150113, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).

  • Flow Cytometry - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)
    Flow Cytometry - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)

    Overlay histogram showing HeLa cells stained with unpurified ab45171 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab45171, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Western blot - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)
    Western blot - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)
    All lanes : Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171) at 1/1000 dilution

    Lane 1 : Recombinant Human ROCK1 protein (aa 1114 to 1354) (30 kDa)
    Lane 2 : Recombinant Human ROCK2 protein (aa 1132 to 1388) (30 kDa)

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 158 kDa
    Observed band size: 30 kDa
    why is the actual band size different from the predicted?



    Loading control: Anti-6X His tag® antibody [EPR20547] (ab213204)

    Blocking buffer and concentration: 5% NFDM/TBST

    Exposure Times:

    Lane 1: 3 seconds

    Lane 2: 7 seconds

  • Immunoprecipitation - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)
    Immunoprecipitation - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)

    ab45171 (purified) at 1/40 immunoprecipitating ROCK2 + ROCK1 in HeLa cell lysate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)
    Western blot - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)
    All lanes : Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171) at 1/5000 dilution (purified)

    Lane 1 : PC-12 cell lysate
    Lane 2 : RAW264.7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 158 kDa
    Observed band size: 158 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)This image is courtesy of an anonymous Abreview

    Unpurified ab45171 staining ROCK2 + ROCK1 in mouse embyro 18dpc tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% FBS/BSA for 2 hours at room temperature; antigen retrieval was by heat mediation in Tris pH 9. Samples were incubated with primary antibody (1/100 in 1% BSA + 1% FBS in TBS) for 16 hours. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid gland carcinoma tissue labelling ROCK2 + ROCK1 with unpurified ab45171.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)
    Anti-ROCK2 + ROCK1 antibody [EP786Y] (ab45171)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (81)

Publishing research using ab45171? Please let us know so that we can cite the reference in this datasheet.

ab45171 has been referenced in 81 publications.

  • Cho WC  et al. SORBS1 serves a metastatic role via suppression of AHNAK in colorectal cancer cell lines. Int J Oncol 56:1140-1151 (2020). PubMed: 32319594
  • Chen C  et al. Ezrin T567 phosphorylation regulates migration and invasion of ectopic endometrial stromal cells by changing actin cytoskeleton. Life Sci 254:117681 (2020). PubMed: 32380081
  • Fan XD  et al. miR-154-3p and miR-487-3p synergistically modulate RHOA signaling in the carcinogenesis of thyroid cancer. Biosci Rep 40:N/A (2020). PubMed: 31820783
  • Wang Z  et al. miR-106b-5p contributes to the lung metastasis of breast cancer via targeting CNN1 and regulating Rho/ROCK1 pathway. Aging (Albany NY) 12:1867-1887 (2020). PubMed: 31986487
  • Hu J  et al. ROCK1 activation-mediated mitochondrial translocation of Drp1 and cofilin are required for arnidiol-induced mitochondrial fission and apoptosis. J Exp Clin Cancer Res 39:37 (2020). PubMed: 32075676
View all Publications for this product

Customer reviews and Q&As

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1-10 of 11 Q&A

Question

Hi I understand, so can we try the different antibody ab143181 please.



Thanks

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Answer

Thank you for confirming these details and for your cooperation.

As requested, I have issued a free of charge replacement of alternative ab134181 Anti-ROCK1 antibody [EPR638Y]with the order number #####.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to letme know. I sincerely hope this vial will work in your experiments. If not, please do not hesitate to contact me and I suggest in this case the best way to proceed would be to provide a refund or credit note for the original antibody.

I wish you the best of luck and look forward to hearing from you with the results of the experiments with the new antibody.

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Question

Hi following on from the email correspondence below, can you let me know if you have a new parent lot yet for ab45171? I really need to get the antibody working again. If not can I try an aliquot of ab134181 which appears to be a new ROCK1 antibody

Thanks

Read More
Answer

Thank you once again for your message. Thank you also for your patience waiting for a response, I wanted to ensure I had time to review the whole case again fully before contacting you as it has been a while since we last corresponded.

I am sorry to confirm that we still only havethe same parent lot ofab45171ROCK1 antibody [EP786Y]available. I would like to reassure you that we monitor feedback closely on a weekly basis and we are not currently concerned about the general quality of this antibody or this batch. Many customers are purchasing this product and when I checked the records,we have received no other complaints in the last year for this product. It is unusual and regrettable that several vials have not worked for you.

I would be pleased to provide a replacement from the same lot, which will still be covered by our guarantee. Alternatively, I fully understand your concerns and you prefer a credit note in this case, or an alternative replacement of ab134181I will be pleased to arrange this for you.

I hope this will be helpful. I look forward to hearing from you with details of how you would like to proceed.

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Question

Hi we have tested the new ROCK1 antibody ab97592 using the same conditions/method we always used for ab45171 when it was working, but unfortunately the new antibody gave just non-specific staining. I was wondering if it was possible to try ab58305 as an alternative to see if this one will work?

Thanks

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Answer

Thank you for your message. I am sorry to hear that the alternative free of charge replacement has also not worked.

It is regrettable both the antibodies have not provided satisfactory results. I would like to reassure you that ab97592 is tested and guaranteed for IHC-P on human samples.

I am concerned that these experiments are still not working for you. In this case, I would like to investigate further Before deciding how to proceed in this case I would appreciate if you are able to confirm the following information which may help me to determine where the difficulty is:

1. Please confirm what the species and tissue typeof the sample. Are these frozen (IHC-Fr) or paraffin embedded sections (IHC-P)?

2. Please confirm how the samples are being processed. Are the samples fresh before fixation? How are they being fixed?

3. What dilution of antibody is being used? Has this been optimized?

4. How is the antibody being stored?

5. Please confirm the wash steps being used?

6. Is the current vial of secondary antibody working well with other primary antibodies? Please confirm the details for the primary antibody. Has a no primary control been included?

Many thanks for your continued cooperation. I look forward to hearing from you with the requested information.

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Question

I think I wrote it in the wrong way,
I would like to test an antibody that can detect ROCK (PCP pathway) in westerns and immuno staining. I does not need to inhibit the signalling itself.
Which one can you recommend?

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Answer

Thank you for contacting us.

All the antibodies are well characterized, please choose based on your requirements. e.g polyclonal/ monoclonal, Rabbit/mouse/sheep etc.

The catalogue numbers are:

ab13660, ab45171, ab56661, ab58305, ab125025

ab71598, ab66310, ab10549, ab97592, ab85612

When selecting check the Abreview and choose the antibody which have more Abreview and publications.

You may also interested in testing the antibody. Please click the below link for more info.

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

I have been using some of antibodies a lot for Immuno-Staining and Western Blotting.
At the moment I am interested in looking at a functional ROCK antibody and its regulation during PCP / Wnt signalling.
Is there an antibody in your product catalogue you can recommend ?
Thanks for your help,
Constanze

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Answer

Thank you for contacting us.

We unfortunately do not have anti ROCK antibodies available that can be used for functional assays e.g. activation or inhibition of ROCK. I would suggest searching the similar antibody clone in publications. We have 6-7 monoclonal antibodies in catalogue however we do not know whether these are being used in any functional assay. I am sorry we are unable to help with this particular ab.

I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

Hi thanks for your message. I would like to try antibody ab97592 please. Fingers crossed that might work! Thanks for your help.

Read More
Answer

Thank you for confirming these details and for your cooperation.

I am sorry that ab45171 has not been working for you and for the inconvenience this has caused. As requested, I have issued an alternative free of charge replacement of 97592 with the order number 1060448.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to letme know.

As previously discussed, I can provide one further free vial if this one works for you. If not, I can provide a credit note or refund on your original purchase.

I wish you the best of luck with your experiments and hope ab97592 proves more successful. Please do not hesitate tocontact me with details of the results. I would be pleased to hearhow you get on.

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Question

Hi thanks for your reply, was the original lot I bought in 2010, lot 873355, from a different parent lot? There is no variation in the frozen sections or reagents I used for the experiment as I set up 3 slides at the same time, one with lot 873355 which worked really well as normal, but GR55792-1 and GR68907-1 gave no expression at all, so the only variation was the aliquot of ROCK1 primary antibody. When will you be getting a different parent lot? Would it be possible to try an aliquot of ab97592 to see if this works? Also it would be great to have a refund for the aliquots we have bought which haven't worked. I really need a good ROCK1 antibody as it is essential to my research!



Thanks for your help



Helen Phillips






Dr Helen M Phillips, PhD
BHF Intermediate Fellow
Institute of Genetic Medicine (Room G227A)
Newcastle University
International Centre for Life
Central Parkway
Newcastle upon Tyne
NE1 3BZ
United Kingdom

Email: Helen.Phillips@ncl.ac.uk


Telephone: ++44 191 241-8698
Fax: ++44 191 241-8666
web: http://www.ncl.ac.uk/ihg








From: technical@abcam.com [mailto:technical@abcam.com]
Sent: 22 March 2012 11:46 PM
To: Helen Phillips
Subject: Reply from Abcam to your enquiry regarding ab45171 [CCE3623937]



















https://www.abcam.com/index.html?utm_campaign=CRM&utm_source=Abcam.CRM&utm_medium=Email&utm_term=Header









Dear Dr Phillips

Thank you for bringing to our attention the issue of variability among the results you have obtained with different vials of the Anti-ROCK1 antibody ab45171.

What I found, after looking at the lots of the threevials that we sent to to Newcastle University, Institute of Genetic Medicine (I am assuming this is affiliated with your lab, or contains it), was that the three vials are all from the same "parent" lot. Thelot numbers on the vialsdiffer according to when the aliquots were drawn from the parent lot.

So the variability is not due to any difference in production, as they are all from the same lot, but there could still be differences introduced by handling, or storage of the parent lot over time (though this is unlikely).

Are there any differences in your other reagents or the samples themselves compared to the successful assays? I am assuming not, so I can either issue another replacement of ab45171 (we still only have the same parent lot), or a vial of another ROCK1 antibody, or a refund for the initial purchase. Have you considered any of our other ROCK1 antibodies, for instance ab97592?

https://www.abcam.com/index.html?datasheet=97592 (or use the following: https://www.abcam.com/index.html?datasheet=97592).

It has not been tested for IHC on frozen tissue, only paraffin-embedded, and reactivity with mouse samples is only predicted, not validated, based on homology of the immunogen with mouse ROCK1. Though not ideal, the other choicesamong our ROCK1 antibodies are less appealing, as none have been tested for IHC of any kind.




Help us improve our service.
https://www.abcam.com/index.html?pageConfig=technicalSurvey&intCCEID=3623937


Best regards,
Thomas

Thomas Ruyle
Scientific Support Specialist
Abcam Inc.
https://www.abcam.com


Your original inquiry to Abcam:





Dear all,







I spoke yesterday to Helen Phillips at the Practicalities of Cellular Analysis using Flow Cytometry, Light and Electron Microscopy workshop in Newcastle about her problems with ab45171. She uses the antibody for doing IHC-Fr on mouse embryonic cryo sections. The customer had an initial lot that worked and purchased a second lot that failed. She contacted customer support and was sent a replacement that is still not working. Do we have any alternative products or do we have a new lot in?







Could you please contact Helen Phillips at mailto:helen.phillips@ncl.ac.uk?







Thanks a lot,







Judith









Abcam Customer Services and Scientific Support Team
https://www.abcam.com/index.html?pageconfig=technical&utm_campaign=CRM&utm_source=Abcam.CRM&utm_medium=Email&utm_term=Body

[CCE3623937]













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Answer

Thank you for your reply, which has been forwarded to me by my colleague Thomas as I was originally dealing with this case.

I am sorry to hear you have had difficulty with the replacementvials.I am concerned that you have used severalvials of ab45171 from the same lot number, and am currently investigating this with our source.I will sendon any further information as soon as I receive it.

In this case, I would be pleased to provide one vial of alternative ab97592 as a free of charge replacement to try. I hope this willwork for you, if it does we can then arrange 1 more free vial.

If ab97592 does not work, we can refund you or provide a credit note for the value of the 2 vials of ab45171 you originally purchased.

I hope this provides a satisfactory solution for the moment. I look forward to hearing from you with details of how you would like to proceed.

Read More

Question

A customer is using the antibody for doing IHC-Fr on mouse embryonic cryo sections. The customer had an initial lot that worked and purchased a second lot that failed. She contacted customer support and was sent a replacement that is still not working. Do we have any alternative products or do we have a new lot in?

Read More
Answer

Thank you for bringing to our attention the issue of variability among the results you have obtained with different vials of the Anti-ROCK1 antibody ab45171.

What I found, after looking at the lots of the three vials that we sent to to your lab, was that the three vials are all from the same "parent" lot. The lot numbers on the vials differ according to when the aliquots were drawn from the parent lot.

So the variability is not due to any difference in production, as they are all from the same lot, but there could still be differences introduced by handling, or storage of the parent lot over time (though this is unlikely).

Are there any differences in your other reagents or the samples themselves compared to the successful assays? I am assuming not, so I can either issue another replacement of ab45171 (we still only have the same parent lot), or a vial of another ROCK1 antibody, or a refund for the initial purchase. Have you considered any of our other ROCK1 antibodies, for instance ab97592?

Click here (or use the following: https://www.abcam.com/index.html?datasheet=97592).

It has not been tested for IHC on frozen tissue, only paraffin-embedded, and reactivity with mouse samples is only predicted, not validated, based on homology of the immunogen with mouse ROCK1. Though not ideal, the other choices among our ROCK1 antibodies are less appealing, as none have been tested for IHC of any kind.

Read More

Question

Would like to try ab121341 with rhesus samples.

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Answer

Thank you for your call today. Please see below forthe code to try ab121341 with rhesus samples, and let me know if you have any questions. Have a great day!

DISCOUNT CODE: ***
Expiration date: June 28, 2012

This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forab121341 with rhesus samplesand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Question

Hi neither of these vials have worked. We'd tried using the first at a higher concentration and hence used it quickly so hence ordered another one quite quickly but didn't have any success with either. It was only when I found an old aliquot of the old lot and did a comparison I realised that it was the antibody that wasn't working and not anything that the students were doing wrong!

Thanks

Read More
Answer

Thank you for confirming these details.

I am sorry the two vials from this lot did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issuedtwofree of charge replacement vials from a different lot:

Order number: ######


To check the status of the order please contact our Customer Service team and reference this number.

Generally, we would encourage customers to contact us as soon as they have difficulty with a product so we can fully investigate before they purchase another vial. On this occasion, we are pleased to make an exception. However, please do not hesitate to contact us as soon as possible in future if you have any difficulties. We are pleased toprovide assistance.

Please note that the free of charge replacement vials are also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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1-10 of 11 Q&A

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