Application
Western blot
Sample
Pig Cell lysate - whole cell (heart)
Gel Running Conditions
Reduced Denaturing
Loading amount
5 µg
Specification
heart
Blocking step
Licor as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 24°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Dilution
1/15000
Name
Non-Abcam antibody was used: IRDye® 800CW Secondary Antibodies
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
Licor imaging
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 158 kDa
Abcam user community
Verified customer
Submitted Nov 04 2016