Anti-ROCK2 (phospho S1366) antibody (ab228008)
Key features and details
- Rabbit polyclonal to ROCK2 (phospho S1366)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-ROCK2 (phospho S1366) antibody
See all ROCK2 primary antibodies -
Description
Rabbit polyclonal to ROCK2 (phospho S1366) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Rhesus monkey -
Immunogen
Synthetic peptide within Human ROCK2 (C terminal) (phospho S1366). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: O75116 -
Positive control
- WB: HEK-293T and HeLa whole cell extracts; HEK-293T transfected with WT ROCK2 whole cell lysate. IHC-P: Human breast tissue; Mouse brain tissue; Rat kidney tissue; HBL435 xenograft tissue. ICC/IF: HeLa cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228008 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 161 kDa.
|
|
IHC-P |
1/100 - 1/1000.
|
|
ICC/IF |
1/100 - 1/1000.
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 161 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
Target
-
Function
Regulates the assembly of the actin cytoskeleton. Promotes formation of stress fibers and of focal adhesion complexes. Plays a role in smooth muscle contraction. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 REM (Hr1) repeat. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. Cell membrane. Cytoplasmic, and associated with actin microfilaments and the plasma membrane. - Information by UniProt
-
Database links
- Entrez Gene: 282041 Cow
- Entrez Gene: 9475 Human
- Entrez Gene: 19878 Mouse
- Entrez Gene: 25537 Rat
- Entrez Gene: 708444 Rhesus monkey
- Omim: 604002 Human
- SwissProt: Q28021 Cow
- SwissProt: O75116 Human
see all -
Alternative names
- coiled-coil-containing protein kinase 2 antibody
- KIAA0619 antibody
- p164 ROCK 2 antibody
see all
Images
-
All lanes : Anti-ROCK2 (phospho S1366) antibody (ab228008) at 1/500 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lanes 2-3 : ROCK2 shRNA transfected HEK-293T whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 161 kDa5% SDS-PAGE gel.
-
All lanes : Anti-ROCK2 (phospho S1366) antibody (ab228008) at 1/2000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract treated with CIP (calf-intestinal alkaline phosphatase)
Lysates/proteins at 30 µg per lane.
Predicted band size: 161 kDa5% SDS-PAGE gel.
-
All lanes : Anti-ROCK2 (phospho S1366) antibody (ab228008) at 1/1000 dilution
Lanes 1 & 3 & 5 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with WT ROCK2, whole cell lysate
Lanes 2 & 4 & 6 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with phospho-mutant (S1366A) ROCK2, whole cell lysate
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 161 kDaHEK-293T cells were transfected with wild type (WT) or phospho-mutant (S1366A) ROCK2 expression constructs. Overexpressed Myc-ROCK2 proteins were immunoprecipitated with anti-myc antibody and probed with ab228008 with or without peptide (0.2 mg/ml) competition as indicated.
-
Paraffin-embedded HBL435 xenograft tissue stained for ROCK2 (phospho S1366) using ab228008 at 1/500 dilution in immunohistochemical analysis.
-
Paraffin-embedded human breast tissue stained for ROCK2 (phospho S1366) using ab228008 at 1/500 dilution in immunohistochemical analysis.
-
Paraffin-embedded mouse brain tissue stained for ROCK2 (phospho S1366) using ab228008 at 1/500 dilution in immunohistochemical analysis.
-
Paraffin-embedded rat kidney tissue stained for ROCK2 (phospho S1366) using ab228008 at 1/500 dilution in immunohistochemical analysis.
-
Ice-cold methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ROCK2 (phospho S1366) (green) using ab228008 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (4)
ab228008 has been referenced in 4 publications.
- Fu A et al. Tumor-resident intracellular microbiota promotes metastatic colonization in breast cancer. Cell 185:1356-1372.e26 (2022). PubMed: 35395179
- Ju J et al. Optical regulation of endogenous RhoA reveals selection of cellular responses by signal amplitude. Cell Rep 40:111080 (2022). PubMed: 35830815
- Baharani A et al. A Progressive Loss of phosphoSer138-Profilin Aligns with Symptomatic Course in the R6/2 Mouse Model of Huntington's Disease: Possible Sex-Dependent Signaling. Cell Mol Neurobiol N/A:N/A (2020). PubMed: 33108594
- Yang S et al. Retracted Article: MiR-182-5p and miR-96-5p increased hepatocellular carcinoma cell mobility, proliferation and cisplatin resistance partially by targeting RND3. RSC Adv 8:34973-34983 (2018). PubMed: 35547072