Anti-ROCK2 (phospho Y722) antibody (ab182648)
Key features and details
- Rabbit polyclonal to ROCK2 (phospho Y722)
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-ROCK2 (phospho Y722) antibody
See all ROCK2 primary antibodies -
Description
Rabbit polyclonal to ROCK2 (phospho Y722) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Synthetic peptide corresponding to Human ROCK2 (phospho Y722) conjugated to Keyhole Limpet Haemocyanin (KLH). Peptide sequence around phosphorylation site of Tyrosine 722 (K-I-Y(p)-E-S) derived from Human ROCK2.
Database link: O75116 -
Positive control
- Rat kidney extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol
PBS (without Mg2+and Ca2+) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab182648 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab182648 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 161 kDa.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 161 kDa. |
Target
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Function
Regulates the assembly of the actin cytoskeleton. Promotes formation of stress fibers and of focal adhesion complexes. Plays a role in smooth muscle contraction. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 REM (Hr1) repeat. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. Cell membrane. Cytoplasmic, and associated with actin microfilaments and the plasma membrane. - Information by UniProt
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Database links
- Entrez Gene: 25537 Rat
- SwissProt: Q62868 Rat
- Unigene: 88642 Rat
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Alternative names
- coiled-coil-containing protein kinase 2 antibody
- KIAA0619 antibody
- p164 ROCK 2 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab182648 has been referenced in 5 publications.
- Zhang X et al. Acquired temozolomide resistance in MGMTlow gliomas is associated with regulation of homologous recombination repair by ROCK2. Cell Death Dis 13:138 (2022). PubMed: 35145081
- Xie L et al. MYO1B enhances colorectal cancer metastasis by promoting the F-actin rearrangement and focal adhesion assembly via RhoA/ROCK/FAK signaling. Ann Transl Med 9:1543 (2021). PubMed: 34790749
- Zhang X et al. Fasudil increases temozolomide sensitivity and suppresses temozolomide-resistant glioma growth via inhibiting ROCK2/ABCG2. Cell Death Dis 9:190 (2018). PubMed: 29416017
- Chen J et al. A role for ErbB signaling in the induction of reactive astrogliosis. Cell Discov 3:17044 (2017). PubMed: 29238610
- Gu Y et al. Fasudil attenuates soluble fms-like tyrosine kinase-1 (sFlt-1)-induced hypertension in pregnant mice through RhoA/ROCK pathway. Oncotarget 8:104104-104112 (2017). PubMed: 29262624