Recombinant Anti-ROGDI antibody [EPR15190] (ab184954)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15190] to ROGDI
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ROGDI antibody [EPR15190]
See all ROGDI primary antibodies -
Description
Rabbit monoclonal [EPR15190] to ROGDI -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal brain and Human bone marrow lysates; HACAT cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15190 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184954 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/10000 - 1/50000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
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Flow Cyt (Intra) |
1/50.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). |
Flow Cyt (Intra)
1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
May act as a positive regulator of cell proliferation. -
Sequence similarities
Belongs to the rogdi family. - Information by UniProt
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Database links
- Entrez Gene: 79641 Human
- Entrez Gene: 66049 Mouse
- Entrez Gene: 287061 Rat
- Omim: 614574 Human
- SwissProt: Q9GZN7 Human
- SwissProt: Q3TDK6 Mouse
- SwissProt: Q4V7D2 Rat
- Unigene: 459795 Human
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Alternative names
- FLJ22386 antibody
- KTZS antibody
- Leucine zipper domain protein antibody
see all
Images
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All lanes : Anti-ROGDI antibody [EPR15190] (ab184954) at 1/20000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human bone marrow lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 32 kDa
Observed band size: 32 kDa -
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixedHACAT cells labeling ROGDI with ab184954 at 1/50 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab184954 has not yet been referenced specifically in any publications.