Overview

  • Product name

    Anti-ROGDI antibody [EPR15190]
    See all ROGDI primary antibodies
  • Description

    Rabbit monoclonal [EPR15190] to ROGDI
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ROGDI aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9GZN7

  • Positive control

    • Human fetal brain and Human bone marrow lysates; HACAT cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15190
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab184954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

Images

  • All lanes : Anti-ROGDI antibody [EPR15190] (ab184954) at 1/20000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human bone marrow lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 32 kDa
    Observed band size: 32 kDa

  • Flow cytometric analysis of 2% paraformaldehyde-fixed HACAT cells labeling ROGDI with ab184954 at 1/50 dilution (red) compared to a Rabbit  monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

References

ab184954 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab184954.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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