Product nameAnti-ROK1 antibody
See all ROK1 primary antibodies
DescriptionRabbit polyclonal to ROK1
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla
Synthetic peptide within Human ROK1 aa 549-599. The exact sequence is proprietary. NP_008941.2
Database link: Q9Y2R4
- WB: HeLa and HEK-293T whole cell lysate. IP: HeLa whole cell lysate.
Previously labelled as DDX52.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab225708 in the following tested applications.
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 67 kDa.|
Sequence similaritiesBelongs to the DEAD box helicase family. DDX52/ROK1 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus > nucleolus.
- Information by UniProt
- ATP dependent RNA helicase ROK1 like antibody
- ATP-dependent RNA helicase ROK1-like antibody
- DDX52 antibody
All lanes : Anti-ROK1 antibody (ab225708) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 67 kDa
Exposure time: 30 seconds
Whole cell lysate from HeLa (human epithelial cell line from cervix adenocarcinoma) cells (1 mg for IP, 20% of IP loaded). ab225708 used for for WB at 0.4 µg/mg and IP at 6 µg/mg lysate.
Lane 1: ab225708 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence 3 seconds.
ab225708 has not yet been referenced specifically in any publications.