Key features and details
- Rabbit polyclonal to ROK1
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ROK1 antibody
See all ROK1 primary antibodies
DescriptionRabbit polyclonal to ROK1
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla
Synthetic peptide within Human ROK1 aa 549-599. The exact sequence is proprietary. NP_008941.2
Database link: Q9Y2R4
- WB: HeLa and HEK-293T whole cell lysate. IP: HeLa whole cell lysate.
Previously labelled as DDX52.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab225708 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 67 kDa.|
Sequence similaritiesBelongs to the DEAD box helicase family. DDX52/ROK1 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus > nucleolus.
- Information by UniProt
- ATP dependent RNA helicase ROK1 like antibody
- ATP-dependent RNA helicase ROK1-like antibody
- DDX52 antibody
All lanes : Anti-ROK1 antibody (ab225708) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 67 kDa
Exposure time: 30 seconds
Whole cell lysate from HeLa (human epithelial cell line from cervix adenocarcinoma) cells (1 mg for IP, 20% of IP loaded). ab225708 used for for WB at 0.4 µg/mg and IP at 6 µg/mg lysate.
Lane 1: ab225708 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence 3 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab225708 has not yet been referenced specifically in any publications.