Overview

  • Product name

  • Description

    Rabbit polyclonal to ROK1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide within Human ROK1 aa 549-599. The exact sequence is proprietary. NP_008941.2
    Database link: Q9Y2R4

  • Positive control

    • WB: HeLa and HEK-293T whole cell lysate. IP: HeLa whole cell lysate.
  • General notes

    Previously labelled as DDX52. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab225708 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 67 kDa.

Target

  • Sequence similarities

    Belongs to the DEAD box helicase family. DDX52/ROK1 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus > nucleolus.
  • Information by UniProt
  • Database links

  • Alternative names

    • ATP dependent RNA helicase ROK1 like antibody
    • ATP-dependent RNA helicase ROK1-like antibody
    • DDX52 antibody
    • DDX52_HUMAN antibody
    • DEAD (Asp-Glu-Ala-Asp) box polypeptide 52 antibody
    • DEAD box protein 52 antibody
    • Human sequence similar to yeast 19 antibody
    • HUSSY19 antibody
    • Probable ATP dependent RNA helicase DDX52 antibody
    • Probable ATP-dependent RNA helicase DDX52 antibody
    • ROK1 antibody
    • ROK1, S. cerevisiae, homolog of antibody
    see all

Images

  • All lanes : Anti-ROK1 antibody (ab225708) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
    Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 67 kDa


    Exposure time: 30 seconds
  • Whole cell lysate from HeLa (human epithelial cell line from cervix adenocarcinoma) cells (1 mg for IP, 20% of IP loaded). ab225708 used for for WB at 0.4 µg/mg and IP at 6 µg/mg lysate.

    Lane 1: ab225708 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection: Chemiluminescence 3 seconds.

References

ab225708 has not yet been referenced specifically in any publications.

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