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Cell Biology Cell Cycle Cell Cycle Inhibitors Other
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Anti-ROMO1 antibody [OTI2C12] (ab236409)

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Western blot - Anti-ROMO1 antibody [OTI2C12] (ab236409)
  • Western blot - Anti-ROMO1 antibody [OTI2C12] (ab236409)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)
  • Immunocytochemistry/ Immunofluorescence - Anti-ROMO1 antibody [OTI2C12] (ab236409)
  • Immunocytochemistry/ Immunofluorescence - Anti-ROMO1 antibody [OTI2C12] (ab236409)

Key features and details

  • Mouse monoclonal [OTI2C12] to ROMO1
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Dog, Human, African green monkey
  • Isotype: IgG1

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Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

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Overview

  • Product name

    Anti-ROMO1 antibody [OTI2C12]
  • Description

    Mouse monoclonal [OTI2C12] to ROMO1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Dog, Human, African green monkey
    Predicted to work with: Cow, Pig
  • Immunogen

    Recombinant full length protein corresponding to Human ROMO1 aa 1 to the C-terminus. (NP_542786) produced in HEK-293T cells.
    Database link: P60602

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: HepG2, HeLa, SVT2, A549, COS-7, Jurkat and MDCK whole cell lysates; pCMV6-ENTRY ROMO1 transfected HEK-293T whole cell lysate. IHC-P: Human breast, breast adenocarcinoma, colon adenocarcinoma, ovary adenocarcinoma, endometrial adenocarcinoma, normal pancreas, thyroid carcinoma, normal prostate and normal kidney tissue. ICC/IF: COS-7 and HeLa cells.
  • General notes

    Clone OTI2C12 (formerly 2C12)

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA, 50% Glycerol
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    Purified from cell culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    OTI2C12
  • Isotype

    IgG1
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Other
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • Other
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Cycle Inhibitors
    • Other
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Cancer
    • Cell cycle
    • Cell division
    • Microbiology
    • Antimicrobial
    • Antibiotic
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • Hep G2 whole cell lysate (ab166833)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • Jurkat whole cell lysate (ab7899)
    • A549 whole cell lysate (ab7910)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab236409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/2000. Predicted molecular weight: 8 kDa.
ICC/IF
1/100.
IHC-P
1/150.

Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes

Notes
WB
1/2000. Predicted molecular weight: 8 kDa.
ICC/IF
1/100.
IHC-P
1/150.

Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes

Target

  • Function

    Induces production of reactive oxygen species (ROS) which are necessary for cell proliferation. May play a role in inducing oxidative DNA damage and replicative senescence.
  • Tissue specificity

    Up-regulated in a number of cancer cell lines when compared to a normal lung fibroblast cell line.
  • Sequence similarities

    Belongs to the MGR2 family.
  • Developmental stage

    Expression increases in senescent cells.
  • Cellular localization

    Mitochondrion membrane.
  • Target information above from: UniProt accession P60602 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 140823 Human
    • Entrez Gene: 67067 Mouse
    • SwissProt: Q3SZV8 Cow
    • SwissProt: P60602 Human
    • SwissProt: P60603 Mouse
    • SwissProt: A1XQR6 Pig
    • Unigene: 472564 Human
    • Unigene: 371623 Mouse
    • Alternative names

      • bA353C18.2 antibody
      • C20orf52 antibody
      • Mitochondrial targeting GXXXG protein antibody
      • MTGMP antibody
      • Protein MGR2 homolog antibody
      • Reactive oxygen species modulator 1 antibody
      • ROMO1 antibody
      • ROMO1_HUMAN antibody
      • ROS modulator 1 antibody
      see all

    Images

    • Western blot - Anti-ROMO1 antibody [OTI2C12] (ab236409)
      Western blot - Anti-ROMO1 antibody [OTI2C12] (ab236409)
      All lanes : Anti-ROMO1 antibody [OTI2C12] (ab236409) at 1/2000 dilution

      Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY control, whole cell lysate
      Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY ROMO1, whole cell lysate

      Lysates/proteins at 5 µg per lane.

      Predicted band size: 8 kDa

    • Western blot - Anti-ROMO1 antibody [OTI2C12] (ab236409)
      Western blot - Anti-ROMO1 antibody [OTI2C12] (ab236409)
      All lanes : Anti-ROMO1 antibody [OTI2C12] (ab236409) at 1/2000 dilution

      Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
      Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 3 : SV-T2 (Mouse cell line) whole cell lysate
      Lane 4 : A549 (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate
      Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
      Lane 7 : MDCK (canine kidney cell line) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 8 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)

      Paraffin-embedded human breast tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)

      Paraffin-embedded human breast adenocarcinoma tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)

      Paraffin-embedded human colon adenocarcinoma tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)

      Paraffin-embedded human kidney tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)

      Paraffin-embedded human ovary adenocarcinoma tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)

      Paraffin-embedded human pancreas tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)

      Paraffin-embedded human thyroid tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)

      Paraffin-embedded human endometrial adenocarcinoma tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)

      Paraffin-embedded human prostate tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.

    • Immunocytochemistry/ Immunofluorescence - Anti-ROMO1 antibody [OTI2C12] (ab236409)
      Immunocytochemistry/ Immunofluorescence - Anti-ROMO1 antibody [OTI2C12] (ab236409)

      COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY ROMO1 stained for ROMO1 (green) using ab236409 at 1/100 dilution in ICC/IF. 

    • Immunocytochemistry/ Immunofluorescence - Anti-ROMO1 antibody [OTI2C12] (ab236409)
      Immunocytochemistry/ Immunofluorescence - Anti-ROMO1 antibody [OTI2C12] (ab236409)

      HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ROMO1 (green) using ab236409 at 1/100 dilution in ICC/IF.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab236409? Please let us know so that we can cite the reference in this datasheet.

    ab236409 has not yet been referenced specifically in any publications.

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