Anti-ROMO1 antibody [OTI2C12] (ab236409)
Key features and details
- Mouse monoclonal [OTI2C12] to ROMO1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Dog, Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-ROMO1 antibody [OTI2C12] -
Description
Mouse monoclonal [OTI2C12] to ROMO1 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Dog, Human, African green monkey
Predicted to work with: Cow, Pig -
Immunogen
Recombinant full length protein corresponding to Human ROMO1 aa 1 to the C-terminus. (NP_542786) produced in HEK-293T cells.
Database link: P60602 -
Positive control
- WB: HepG2, HeLa, SVT2, A549, COS-7, Jurkat and MDCK whole cell lysates; pCMV6-ENTRY ROMO1 transfected HEK-293T whole cell lysate. IHC-P: Human breast, breast adenocarcinoma, colon adenocarcinoma, ovary adenocarcinoma, endometrial adenocarcinoma, normal pancreas, thyroid carcinoma, normal prostate and normal kidney tissue. ICC/IF: COS-7 and HeLa cells.
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General notes
Clone OTI2C12 (formerly 2C12)
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
OTI2C12 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236409 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000. Predicted molecular weight: 8 kDa.
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ICC/IF |
1/100.
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IHC-P |
1/150.
Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes |
Notes |
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WB
1/2000. Predicted molecular weight: 8 kDa. |
ICC/IF
1/100. |
IHC-P
1/150. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes |
Target
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Function
Induces production of reactive oxygen species (ROS) which are necessary for cell proliferation. May play a role in inducing oxidative DNA damage and replicative senescence. -
Tissue specificity
Up-regulated in a number of cancer cell lines when compared to a normal lung fibroblast cell line. -
Sequence similarities
Belongs to the MGR2 family. -
Developmental stage
Expression increases in senescent cells. -
Cellular localization
Mitochondrion membrane. - Information by UniProt
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Database links
- Entrez Gene: 140823 Human
- Entrez Gene: 67067 Mouse
- SwissProt: Q3SZV8 Cow
- SwissProt: P60602 Human
- SwissProt: P60603 Mouse
- SwissProt: A1XQR6 Pig
- Unigene: 472564 Human
- Unigene: 371623 Mouse
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Alternative names
- bA353C18.2 antibody
- C20orf52 antibody
- Mitochondrial targeting GXXXG protein antibody
see all
Images
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All lanes : Anti-ROMO1 antibody [OTI2C12] (ab236409) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY control, whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY ROMO1, whole cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 8 kDa -
All lanes : Anti-ROMO1 antibody [OTI2C12] (ab236409) at 1/2000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : SV-T2 (Mouse cell line) whole cell lysate
Lane 4 : A549 (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 7 : MDCK (canine kidney cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 8 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)
Paraffin-embedded human breast tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)
Paraffin-embedded human breast adenocarcinoma tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)
Paraffin-embedded human colon adenocarcinoma tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)
Paraffin-embedded human kidney tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)
Paraffin-embedded human ovary adenocarcinoma tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)
Paraffin-embedded human pancreas tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)
Paraffin-embedded human thyroid tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)
Paraffin-embedded human endometrial adenocarcinoma tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROMO1 antibody [OTI2C12] (ab236409)
Paraffin-embedded human prostate tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.
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COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY ROMO1 stained for ROMO1 (green) using ab236409 at 1/100 dilution in ICC/IF.
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ROMO1 (green) using ab236409 at 1/100 dilution in ICC/IF.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab236409 has not yet been referenced specifically in any publications.