Recombinant Anti-RON antibody [EP1132Y] (ab52927)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1132Y] to RON
- Suitable for: ELISA, WB, IP, Flow Cyt, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-RON antibody [EP1132Y]
See all RON primary antibodies -
Description
Rabbit monoclonal [EP1132Y] to RON -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, WB, IP, Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human RON aa 1-100 (N terminal). The exact sequence is proprietary.
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Positive control
- SKBR3 cell lysate; human stomach tissue.
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General notes
The major RON transcript is translated into a glycosylated single chain precursor, cleaved into a 185 kDa heterodimer (p185Ron) of 35 (alpha) and 150 kDa (beta) disulfide-linked chains, before exposure at the cell surface.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP1132Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab52927 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA | Use at an assay dependent concentration. | |
WB | 1/10000. Detects a band of approximately 35,150,180 kDa (predicted molecular weight: 185 kDa). 1/10000. Detects a band of approximately 35/150/180 kDa (predicted molecular weight: 185 kDa). See Notes section of Datasheet. | |
IP | 1/30. | |
Flow Cyt | 1/20 - 1/40. Methanol fixed cells. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Receptor for macrophage stimulating protein (MSP). Has a tyrosine-protein kinase activity. -
Tissue specificity
Keratinocytes and lung. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family.
Contains 3 IPT/TIG domains.
Contains 1 protein kinase domain.
Contains 1 Sema domain. -
Post-translational
modificationsProteolytic processing yields the two subunits.
Phosphorylated in response to ligand binding. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 4486 Human
- Omim: 600168 Human
- SwissProt: Q04912 Human
- Unigene: 517973 Human
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Alternative names
- c met related tyrosine kinase antibody
- CD136 antibody
- CD136 antigen antibody
see all
Images
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Anti-RON antibody [EP1132Y] (ab52927) at 1/10000 dilution + SKBR3 cell lysate at 10 µg
Secondary
HRP-labelled goat anti-rabbit
Predicted band size: 185 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RON antibody [EP1132Y] (ab52927)
Immunohistochemical staining of paraffin-embedded human stomach tissue using ab52927 at a dilution of 1/100-1/250.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Overlay histogram showing THP1 cells stained with ab52927 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52927, 1/20 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in THP1 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam do not have any data for use of this antibody in non-fixed cells. We welcome any customer feedback. -
ELISA analysis of Human RON recombinant protein at 1000 ng/mL with ab52927. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (6)
ab52927 has been referenced in 6 publications.
- Hao C et al. OPN promotes the aggressiveness of non-small-cell lung cancer cells through the activation of the RON tyrosine kinase. Sci Rep 9:18101 (2019). PubMed: 31792339
- LoRusso PM et al. Phase 1 study of narnatumab, an anti-RON receptor monoclonal antibody, in patients with advanced solid tumors. Invest New Drugs 35:442-450 (2017). PubMed: 28161886
- Han DH et al. A missing link between RON expression and oncological outcomes in resected left-sided pancreatic cancer. Oncol Lett 14:4225-4230 (2017). PubMed: 28943931
- Zhang Y et al. Plasma Level of Placenta-Derived Macrophage-Stimulating Protein -Chain in Preeclampsia before 20 Weeks of Pregnancy. PLoS One 11:e0161626 (2016). IHC-P . PubMed: 27559727
- Wang Q et al. RON confers lapatinib resistance in HER2-positive breast cancer cells. Cancer Lett 340:43-50 (2013). WB ; Human . PubMed: 23811285
- Mühlebach MD et al. Adherens junction protein nectin-4 is the epithelial receptor for measles virus. Nature 480:530-3 (2011). PubMed: 22048310