Recombinant
RabMAb

Recombinant Anti-RON antibody [EP1132Y] (ab52927)

Reviews (1)Q&A (1)References (6)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1132Y] to RON
  • Suitable for: ELISA, WB, IP, Flow Cyt, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-RON antibody [EP1132Y]
    See all RON primary antibodies

  • Description

    Rabbit monoclonal [EP1132Y] to RON

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ELISA, WB, IP, Flow Cyt, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human RON aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • SKBR3 cell lysate; human stomach tissue.

  • General notes

     

    The major RON transcript is translated into a glycosylated single chain precursor, cleaved into a 185 kDa heterodimer (p185Ron) of 35 (alpha) and 150 kDa (beta) disulfide-linked chains, before exposure at the cell surface.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab52927 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB 1/10000. Detects a band of approximately 35,150,180 kDa (predicted molecular weight: 185 kDa). 1/10000. Detects a band of approximately 35/150/180 kDa (predicted molecular weight: 185 kDa). See Notes section of Datasheet.
IP 1/30.
Flow Cyt 1/20 - 1/40.

Methanol fixed cells.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.

Target

  • Function

    Receptor for macrophage stimulating protein (MSP). Has a tyrosine-protein kinase activity.

  • Tissue specificity

    Keratinocytes and lung.

  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family.
    Contains 3 IPT/TIG domains.
    Contains 1 protein kinase domain.
    Contains 1 Sema domain.

  • Post-translational
    modifications

    Proteolytic processing yields the two subunits.
    Phosphorylated in response to ligand binding.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • c met related tyrosine kinase antibody
      • CD136 antibody
      • CD136 antigen antibody
      • CDw136 antibody
      • Macrophage stimulating 1 receptor (c met related tyrosine kinase) antibody
      • Macrophage stimulating 1 receptor antibody
      • Macrophage stimulating protein receptor alpha chain antibody
      • MACROPHAGE STIMULATING PROTEIN RECEPTOR antibody
      • Macrophage stimulating protein receptor beta chain antibody
      • Macrophage-Stimulating 1 Receptor (MST1R) antibody
      • Macrophage-stimulating protein receptor beta chain antibody
      • MSP receptor antibody
      • Mst1r antibody
      • MST1R variant RON30 antibody
      • MST1R variant RON62 antibody
      • NPCA3 antibody
      • p185 RON antibody
      • p185-Ron antibody
      • Protein-tyrosine kinase 8 antibody
      • PTK 8 antibody
      • ptk8 antibody
      • PTK8 protein tyrosine kinase 8 antibody
      • Recepteur d’origine nantais (RON) antibody
      • RON antibody
      • RON protein tyrosine kinase antibody
      • RON variant E2E3 antibody
      • RON_HUMAN antibody
      • Soluble RON variant 1 antibody
      • Soluble RON variant 2 antibody
      • Soluble RON variant 3 antibody
      • Soluble RON variant 4 antibody
      • Stem cell derived tyrosine kinase antibody
      see all

    Images

    • Western blot - Anti-RON antibody [EP1132Y] (ab52927)
      Western blot - Anti-RON antibody [EP1132Y] (ab52927)
      Anti-RON antibody [EP1132Y] (ab52927) at 1/10000 dilution + SKBR3 cell lysate at 10 µg

      Secondary
      HRP-labelled goat anti-rabbit

      Predicted band size: 185 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RON antibody [EP1132Y] (ab52927)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RON antibody [EP1132Y] (ab52927)

      Immunohistochemical staining of paraffin-embedded human stomach tissue using ab52927 at a dilution of 1/100-1/250.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-RON antibody [EP1132Y] (ab52927)
      Flow Cytometry - Anti-RON antibody [EP1132Y] (ab52927)
      Overlay histogram showing THP1 cells stained with ab52927 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52927, 1/20 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in THP1 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

      Please note that Abcam do not have any data for use of this antibody in non-fixed cells. We welcome any customer feedback.
    • ELISA - Anti-RON antibody [EP1132Y] (ab52927)
      ELISA - Anti-RON antibody [EP1132Y] (ab52927)
      ELISA analysis of Human RON recombinant protein at 1000 ng/mL with ab52927. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (6)

    Publishing research using ab52927? Please let us know so that we can cite the reference in this datasheet.

    ab52927 has been referenced in 6 publications.

    • Hao C  et al. OPN promotes the aggressiveness of non-small-cell lung cancer cells through the activation of the RON tyrosine kinase. Sci Rep 9:18101 (2019). PubMed: 31792339
    • LoRusso PM  et al. Phase 1 study of narnatumab, an anti-RON receptor monoclonal antibody, in patients with advanced solid tumors. Invest New Drugs 35:442-450 (2017). PubMed: 28161886
    • Han DH  et al. A missing link between RON expression and oncological outcomes in resected left-sided pancreatic cancer. Oncol Lett 14:4225-4230 (2017). PubMed: 28943931
    • Zhang Y  et al. Plasma Level of Placenta-Derived Macrophage-Stimulating Protein -Chain in Preeclampsia before 20 Weeks of Pregnancy. PLoS One 11:e0161626 (2016). IHC-P . PubMed: 27559727
    • Wang Q  et al. RON confers lapatinib resistance in HER2-positive breast cancer cells. Cancer Lett 340:43-50 (2013). WB ; Human . PubMed: 23811285
    • Mühlebach MD  et al. Adherens junction protein nectin-4 is the epithelial receptor for measles virus. Nature 480:530-3 (2011). PubMed: 22048310

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    Is the specific isotype known for this antibody?  Is it IgG1 or IgG2? How was the product validated for IP?

    Read More
    Answer

    Thank you for contacting Abcam regarding ab52927. I have confirmed that this antibody is an IgG1 isotype. Below, please find the IP protocol used: 1. Mix 200–300 μl of non-denatured cell lysate with appropriate dilutions of primary antibody 2. Incubate overnight at 4 °C with gentle rocking. 3. Add 25 μl of Protein G beads (50% bead slurry) and incubate 2 hours at 4 °C with gentle rocking. 4. Microcentrifuge for 30 seconds and discard the supernatant. Wash pellet 3 times with cold PBS. 5. Resuspend the pellet with 20 μl of 2x SDS reducing sample buffer containing β-mercaptoethanol (5%, v/v) and heat the sample at 95–100 °C for 3 minutes. 6. Briefly centrifuge the sample. 7. Load 16 μl of sample on to a SDS-PAGE gel or freeze the sample for later use (reboiled for 3 minutes prior loading onto the SDS-PAGE gel). 8. Perform Western blotting analysis using HRP-conjugated anti-rabbit IgG as secondary antibody  I hope this information is helpful.  Please do not hesitate to contact us if you have any additional questions.

    Read More

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-RON antibody [EP1132Y]

     Average
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Normal Colon)
    Specification
    Normal Colon
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 10 minute(s) · Concentration: 10%
    Read More

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    Submitted May 01 2009

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