Anti-RON antibody [EP1132Y] (ab52927)
- Datasheet
- Specific References (4)
- Protocols
Overview
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Product nameAnti-RON antibody [EP1132Y]
See all RON primary antibodies -
DescriptionRabbit monoclonal [EP1132Y] to RON
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IP, Flow Cyt, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide within Human RON aa 1-100 (N terminal). The exact sequence is proprietary.
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Positive control
- SKBR3 cell lysate; human stomach tissue.
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General notes
The major RON transcript is translated into a glycosylated single chain precursor, cleaved into a 185 kDa heterodimer (p185Ron) of 35 (alpha) and 150 kDa (beta) disulfide-linked chains, before exposure at the cell surface.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEP1132Y
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
Applications
Our Abpromise guarantee covers the use of ab52927 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000. Detects a band of approximately 35,150,180 kDa (predicted molecular weight: 185 kDa). 1/10000. Detects a band of approximately 35/150/180 kDa (predicted molecular weight: 185 kDa). See Notes section of Datasheet. | |
| IP | 1/30. | |
| Flow Cyt | 1/20 - 1/40. Methanol fixed cells. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IHC-P | Use at an assay dependent concentration. | |
| ICC/IF | 1/50 - 1/100. |
Target
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FunctionReceptor for macrophage stimulating protein (MSP). Has a tyrosine-protein kinase activity.
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Tissue specificityKeratinocytes and lung.
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Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family.
Contains 3 IPT/TIG domains.
Contains 1 protein kinase domain.
Contains 1 Sema domain. -
Post-translational
modificationsProteolytic processing yields the two subunits.
Phosphorylated in response to ligand binding. -
Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 4486 Human
- Omim: 600168 Human
- SwissProt: Q04912 Human
- Unigene: 517973 Human
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Alternative names
- c met related tyrosine kinase antibody
- CD136 antibody
- CD136 antigen antibody
see all
Images
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![Western blot - Anti-RON antibody [EP1132Y] (ab52927)](https://www.abcam.com/ps/products/52/ab52927/Images/ab52927wb.jpg)
Western blot - Anti-RON antibody [EP1132Y] (ab52927)Anti-RON antibody [EP1132Y] (ab52927) at 1/10000 dilution + SKBR3 cell lysate at 10 µg
Secondary
HRP-labelled goat anti-rabbit
Predicted band size: 185 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RON antibody [EP1132Y] (ab52927)](https://www.abcam.com/ps/products/52/ab52927/Images/ab52927ihc.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RON antibody [EP1132Y] (ab52927)Immunohistochemical staining of paraffin-embedded human stomach tissue using ab52927 at a dilution of 1/100-1/250. -
![Flow Cytometry - Anti-RON antibody [EP1132Y] (ab52927)](https://www.abcam.com/ps/products/52/ab52927/Images/RON-Primary-antibodies-ab52927-1.jpg)
Flow Cytometry - Anti-RON antibody [EP1132Y] (ab52927)Overlay histogram showing THP1 cells stained with ab52927 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52927, 1/20 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in THP1 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam do not have any data for use of this antibody in non-fixed cells. We welcome any customer feedback.
References
This product has been referenced in:
- Han DH et al. A missing link between RON expression and oncological outcomes in resected left-sided pancreatic cancer. Oncol Lett 14:4225-4230 (2017). Read more (PubMed: 28943931) »
- Zhang Y et al. Plasma Level of Placenta-Derived Macrophage-Stimulating Protein -Chain in Preeclampsia before 20 Weeks of Pregnancy. PLoS One 11:e0161626 (2016). IHC-P . Read more (PubMed: 27559727) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"