Product nameAnti-RON antibody [EP1132Y]
See all RON primary antibodies
DescriptionRabbit monoclonal [EP1132Y] to RON
Tested applicationsSuitable for: WB, IP, Flow Cyt, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide within Human RON aa 1-100 (N terminal). The exact sequence is proprietary.
- SKBR3 cell lysate; human stomach tissue.
The major RON transcript is translated into a glycosylated single chain precursor, cleaved into a 185 kDa heterodimer (p185Ron) of 35 (alpha) and 150 kDa (beta) disulfide-linked chains, before exposure at the cell surface.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab52927 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000. Detects a band of approximately 35,150,180 kDa (predicted molecular weight: 185 kDa). 1/10000. Detects a band of approximately 35/150/180 kDa (predicted molecular weight: 185 kDa). See Notes section of Datasheet.|
|Flow Cyt||1/20 - 1/40.
Methanol fixed cells.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||1/50 - 1/100.|
FunctionReceptor for macrophage stimulating protein (MSP). Has a tyrosine-protein kinase activity.
Tissue specificityKeratinocytes and lung.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family.
Contains 3 IPT/TIG domains.
Contains 1 protein kinase domain.
Contains 1 Sema domain.
modificationsProteolytic processing yields the two subunits.
Phosphorylated in response to ligand binding.
- Information by UniProt
- c met related tyrosine kinase antibody
- CD136 antibody
- CD136 antigen antibody
Anti-RON antibody [EP1132Y] (ab52927) at 1/10000 dilution + SKBR3 cell lysate at 10 µg
HRP-labelled goat anti-rabbit
Predicted band size: 185 kDa
Immunohistochemical staining of paraffin-embedded human stomach tissue using ab52927 at a dilution of 1/100-1/250.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Overlay histogram showing THP1 cells stained with ab52927 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52927, 1/20 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in THP1 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam do not have any data for use of this antibody in non-fixed cells. We welcome any customer feedback.
This product has been referenced in:
- Han DH et al. A missing link between RON expression and oncological outcomes in resected left-sided pancreatic cancer. Oncol Lett 14:4225-4230 (2017). Read more (PubMed: 28943931) »
- LoRusso PM et al. Phase 1 study of narnatumab, an anti-RON receptor monoclonal antibody, in patients with advanced solid tumors. Invest New Drugs 35:442-450 (2017). Read more (PubMed: 28161886) »