Overview

  • Product name

    Anti-RON antibody [EPR20953]
    See all RON primary antibodies
  • Description

    Rabbit monoclonal [EPR20953] to RON
  • Host species

    Rabbit
  • Specificity

    ab227980 detects a distinct immunogen compared to ab227979.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human RON aa 1-200. The exact sequence is proprietary.
    Database link: Q04912

  • Positive control

    • WB: BxPC-3, HCT116, SW480 and SK-BR-3 whole cell lysate; human stomach tissue lysate. IHC: Human stomach, tonsil and bladder cancer tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab227980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 35, 152 kDa (predicted molecular weight: 152 kDa).
IHC-P 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Perform heat mediated antigen retrieval using Universal HIER antigen retrieval reagent (10X) (ab208572).

Target

  • Function

    Receptor for macrophage stimulating protein (MSP). Has a tyrosine-protein kinase activity.
  • Tissue specificity

    Keratinocytes and lung.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family.
    Contains 3 IPT/TIG domains.
    Contains 1 protein kinase domain.
    Contains 1 Sema domain.
  • Post-translational
    modifications

    Proteolytic processing yields the two subunits.
    Phosphorylated in response to ligand binding.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • c met related tyrosine kinase antibody
    • CD136 antibody
    • CD136 antigen antibody
    • CDw136 antibody
    • Macrophage stimulating 1 receptor (c met related tyrosine kinase) antibody
    • Macrophage stimulating 1 receptor antibody
    • Macrophage stimulating protein receptor alpha chain antibody
    • MACROPHAGE STIMULATING PROTEIN RECEPTOR antibody
    • Macrophage stimulating protein receptor beta chain antibody
    • Macrophage-Stimulating 1 Receptor (MST1R) antibody
    • Macrophage-stimulating protein receptor beta chain antibody
    • MSP receptor antibody
    • Mst1r antibody
    • MST1R variant RON30 antibody
    • MST1R variant RON62 antibody
    • NPCA3 antibody
    • p185 RON antibody
    • p185-Ron antibody
    • Protein-tyrosine kinase 8 antibody
    • PTK 8 antibody
    • ptk8 antibody
    • PTK8 protein tyrosine kinase 8 antibody
    • Recepteur d’origine nantais (RON) antibody
    • RON antibody
    • RON protein tyrosine kinase antibody
    • RON variant E2E3 antibody
    • RON_HUMAN antibody
    • Soluble RON variant 1 antibody
    • Soluble RON variant 2 antibody
    • Soluble RON variant 3 antibody
    • Soluble RON variant 4 antibody
    • Stem cell derived tyrosine kinase antibody
    see all

Images

  • All lanes : Anti-RON antibody [EPR20953] (ab227980) at 1/1000 dilution

    Lane 1 : BxPC-3 (human pancreas adenocarcinoma cell line) whole cell lysate at 20 µg
    Lane 2 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 20 µg
    Lane 3 : SW480 (human colorectal adenocarcinoma cell line) whole cell lysate at 10 µg
    Lane 4 : SK-BR-3 (human mammary gland adenocarcinoma cell line) whole cell lysate at 10 µg
    Lane 5 : Human stomach tissue at 10 µg

    Secondary
    Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lanes 3-5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 152 kDa
    Observed band size: 35, 175 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution: 5% NFDm/TBST

    Exposure: Lanes 1-4: 3 minutes; Lane t: 2 minutes.

    The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

    The 185kDa RON precursor protein is cleaved into a 40kDa α and 150kDa β chain, which are linked by disulphide bonds in the 180kDa mature form (PMID: 23792360, PMID: 9890710). ab227980 is specific for the α chain.

  • Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling RON with ab227980 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Cytoplasmic and membranous staining in human gastric epithelium is observed (PMID: 9890710). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling RON with ab227980 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Cytoplasmic and membranous staining in germinal centers of human tonsil is observed (PMID: 9890710). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling RON with ab227980 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Membranous staining on human bladder cancer cells is observed (PMID: 15870710). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

References

ab227980 has not yet been referenced specifically in any publications.

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