Recombinant
RabMAb

Recombinant Anti-RON antibody [EPR20953] - BSA and Azide free (ab228855)

Overview

  • Product name

    Anti-RON antibody [EPR20953] - BSA and Azide free
    See all RON primary antibodies
  • Description

    Rabbit monoclonal [EPR20953] to RON - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    ab227980 detects a distinct immunogen compared to ab227979.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment aa 1-200. The exact sequence is proprietary.
    Database link: Q04912

  • Positive control

    • IHC: Human stomach tissue.
  • General notes

    Ab228855 is the carrier-free version of ab227980. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab228855 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab228855 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 35, 152 kDa (predicted molecular weight: 152 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Perform heat mediated antigen retrieval using Universal HIER antigen retrieval reagent (10X) (ab208572).

Target

  • Function

    Receptor for macrophage stimulating protein (MSP). Has a tyrosine-protein kinase activity.
  • Tissue specificity

    Keratinocytes and lung.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family.
    Contains 3 IPT/TIG domains.
    Contains 1 protein kinase domain.
    Contains 1 Sema domain.
  • Post-translational
    modifications

    Proteolytic processing yields the two subunits.
    Phosphorylated in response to ligand binding.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • c met related tyrosine kinase antibody
    • CD136 antibody
    • CD136 antigen antibody
    • CDw136 antibody
    • Macrophage stimulating 1 receptor (c met related tyrosine kinase) antibody
    • Macrophage stimulating 1 receptor antibody
    • Macrophage stimulating protein receptor alpha chain antibody
    • MACROPHAGE STIMULATING PROTEIN RECEPTOR antibody
    • Macrophage stimulating protein receptor beta chain antibody
    • Macrophage-Stimulating 1 Receptor (MST1R) antibody
    • Macrophage-stimulating protein receptor beta chain antibody
    • MSP receptor antibody
    • Mst1r antibody
    • MST1R variant RON30 antibody
    • MST1R variant RON62 antibody
    • NPCA3 antibody
    • p185 RON antibody
    • p185-Ron antibody
    • Protein-tyrosine kinase 8 antibody
    • PTK 8 antibody
    • ptk8 antibody
    • PTK8 protein tyrosine kinase 8 antibody
    • Recepteur d’origine nantais (RON) antibody
    • RON antibody
    • RON protein tyrosine kinase antibody
    • RON variant E2E3 antibody
    • RON_HUMAN antibody
    • Soluble RON variant 1 antibody
    • Soluble RON variant 2 antibody
    • Soluble RON variant 3 antibody
    • Soluble RON variant 4 antibody
    • Stem cell derived tyrosine kinase antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling RON with ab227980 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Membranous staining on human bladder cancer cells is observed (PMID: 15870710). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227980).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling RON with ab227980 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Cytoplasmic and membranous staining in germinal centers of human tonsil is observed (PMID: 9890710). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227980).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling RON with ab227980 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Cytoplasmic and membranous staining in human gastric epithelium is observed (PMID: 9890710). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227980).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

References

ab228855 has not yet been referenced specifically in any publications.

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