Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5598] to RON (phospho Y1353)
- Suitable for: WB, IP, ICC/IF
- Reacts with: Human
Product nameAnti-RON (phospho Y1353) antibody [EPR5598]
See all RON primary antibodies
DescriptionRabbit monoclonal [EPR5598] to RON (phospho Y1353)
SpecificityOnly detects RON phosphorylated at Tyrosine 1353.
Tested applicationsSuitable for: WB, IP, ICC/IFmore details
Unsuitable for: Flow Cyt or IHC-P
Species reactivityReacts with: Human
Synthetic peptide within Human RON (C terminal) (phospho Y1353). The exact sequence is proprietary.
- A431 cell lysates treated with pervanadate; A431 cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 9.05 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124671 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/50000 - 1/200000. Detects a band of approximately 180 kDa (predicted molecular weight: 152 kDa).|
|IP||1/10 - 1/100.|
|ICC/IF||1/500 - 1/1000.|
FunctionReceptor for macrophage stimulating protein (MSP). Has a tyrosine-protein kinase activity.
Tissue specificityKeratinocytes and lung.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family.
Contains 3 IPT/TIG domains.
Contains 1 protein kinase domain.
Contains 1 Sema domain.
modificationsProteolytic processing yields the two subunits.
Phosphorylated in response to ligand binding.
- Information by UniProt
- c met related tyrosine kinase antibody
- CD136 antibody
- CD136 antigen antibody
All lanes : Anti-RON (phospho Y1353) antibody [EPR5598] (ab124671) at 1/50000 dilution
Lane 1 : A431 cell lysates, untreated
Lane 2 : A431 cell lysates, treated with pervanadate
Predicted band size: 152 kDa
ab124671, at 1/500 dilution, staining RON in A431 (Human epidermoid carcinoma cell line) cells by Immunofluorescence.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab124671 has been referenced in 1 publication.
- de la Fuente C et al. Phosphoproteomic analysis reveals Smad protein family activation following Rift Valley fever virus infection. PLoS One 13:e0191983 (2018). PubMed: 29408900