Anti-Roquin-2 antibody (ab241404)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-Roquin-2 antibody
See all Roquin-2 primary antibodies -
Description
Rabbit polyclonal to Roquin-2 -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Roquin-2 aa 1100-1150. The exact sequence is proprietary.
Database link: Q9HBD1 -
Positive control
- IP: Roquin-2 IP in HeLa whole cell lysate.
-
General notes
ab241404 has not performed satisfactorily when used for WB of Roquin-2 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Roquin-2.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to Roquin-2 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab241404 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-10 µg/mg of lysate. |
Target
-
Tissue specificity
Expressed in spleen, testis, ovary and small intestine. -
Sequence similarities
Contains 1 C3H1-type zinc finger.
Contains 1 RING-type zinc finger. -
Cellular localization
Cytoplasm > perinuclear region. Endomembrane system. Membrane-associated. Primarily localizes to the perinuclear space, probably to the endoplasmic reticulum or trans-Golgi network. - Information by UniProt
-
Database links
- Entrez Gene: 54542 Human
- Omim: 615231 Human
- SwissProt: Q9HBD1 Human
- Unigene: 533499 Human
-
Alternative names
- 2900024N03Rik antibody
- 9430019J22Rik antibody
- D930043C02Rik antibody
see all
Images
-
Immunoprecipitation - Anti-Roquin-2 antibody (ab241404)Roquin-2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241404 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241404 at 0.4 µg/ml.
Lane 1: ab241404 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with exposure time of 3 minutes.
Datasheets and documents
References
ab241404 has not yet been referenced specifically in any publications.
