Key features and details
- Rabbit polyclonal to Roquin-2
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Roquin-2 antibody
See all Roquin-2 primary antibodies
DescriptionRabbit polyclonal to Roquin-2
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide within Human Roquin-2 aa 900-950. The exact sequence is proprietary.
Database link: Q9HBD1
- IP: HeLa whole cell lysate.
ab241406 has not performed satisfactorily when used for WB of Roquin-2 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Roquin-2.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab241406 was affinity purified using an epitope specific to Roquin-2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab241406 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
Tissue specificityExpressed in spleen, testis, ovary and small intestine.
Sequence similaritiesContains 1 C3H1-type zinc finger.
Contains 1 RING-type zinc finger.
Cellular localizationCytoplasm > perinuclear region. Endomembrane system. Membrane-associated. Primarily localizes to the perinuclear space, probably to the endoplasmic reticulum or trans-Golgi network.
- Information by UniProt
- 2900024N03Rik antibody
- 9430019J22Rik antibody
- D930043C02Rik antibody
Roquin-2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab241406 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab241406 at 0.04 µg/ml.
Lane 1: ab241406 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.
Detection: Chemiluminescence with an exposure time of 3 minutes.
Lysates prepared using NETN lysis buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241406 has not yet been referenced specifically in any publications.