Immunohistochemistry (Frozen sections) abreview for Anti-ROR alpha/RORA antibody

Average
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (mouse adult retina)
Permeabilization
Yes - 0.1% triton-x100
Specification
mouse adult retina
Blocking step
10% FBS, 1%BSA as blocking agent for 5 hour(s) and 0 minute(s) · Concentration: NA% · Temperature: 22°C
Fixative
Paraformaldehyde

Other product details

Incubation time
5 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS ph7.4
Dilution
1/100

Secondary antibody

Dilution
1/250
Name
Non-Abcam antibody was used: goat anti rabbit Alexa Fluor 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594

Additional data

Additional Notes
This is a tricky antibody and quality of staining varied between experiments. Fixing the tissue for 1h at 4C in 4%PFA/PBS gave acceptable results.

Abcam user community

Verified customer

Submitted Feb 20 2009

Sign up