Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ROR alpha/RORA antibody

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lung tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6.0
Permeabilization
Yes - 0.05% tween 20
Specification
Lung tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Formaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking buffer
Dilution
1/250

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: Biotinylated Goat Anti-Rabbit IgG Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin

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Submitted Mar 14 2015

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