Western blot abreview for Anti-ROR alpha/RORA antibody

Good
Abreviews
Application
Western blot
Sample
Human Tissue lysate - whole (Human lung)
Gel Running Conditions
Reduced Denaturing (7.5%)
Loading amount
25 µg
Specification
Human lung
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk
Dilution
1/1000

Secondary antibody

Dilution
1/20000
Name
Non-Abcam antibody was used: Goat anti-rabbit HRP IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: ~68 kDa

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Submitted Mar 20 2015

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