Product nameAnti-ROR alpha/RORA antibody
See all ROR alpha/RORA primary antibodies
DescriptionRabbit polyclonal to ROR alpha/RORA
Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human ROR alpha/RORA aa 31-70 (N terminal).
- Human breast carcinoma tissue, extracts from HeLa and COS-7 cells.
This product was previously labelled as ROR alpha
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70061 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).|
|IHC-P||1/50 - 1/100.|
FunctionOrphan nuclear receptor. Binds DNA as a monomer to hormone response elements (HRE) containing a single core motif half-site preceded by a short A-T-rich sequence. This isomer binds to the consensus sequence 5'-[AT][TA]A[AT][CGT]TAGGTCA-3'. Regulates a number of genes involved in lipid metabolism such as apolipoproteins AI, APOA5, CIII, CYP71 and PPARgamma, in cerebellum and photoreceptor development including PCP2, OPN1SW, OPN1SM AND ARR3, in circadian rhythm with BMAL1, and skeletal muscle development with MYOD1. Possible receptor for cholesterol or one of its derivatives.
Tissue specificityWidely expressed in a number of tissues.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
modificationsPhosphorylation by PKC in neurons inhibits transcriptional activity. Phosphorylated on Thr-216 by ERK2 in vitro.
Sumoylated by SENP1 and SENP2. Sumoylation, promoted by PIAS2, PIAS3, PIASy but not PIAS1, enhances the transcriptional activity. Desumoylated by SENP1.
Ubiquitinated. Ubiquitination is required for efficient transcriptional activity and is prevented by Hairless.
- Information by UniProt
- fhl2a antibody
- NR1F1 antibody
- Nuclear receptor ROR alpha antibody
All lanes : Anti-ROR alpha/RORA antibody (ab70061) at 1/500 dilution
Lane 1 : extract from HeLa cells
Lane 2 : extract from COS-7 cells
Lane 3 : extract from COS-7 cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 63 kDa
Observed band size: 63 kDa
Human breast carcinoma tissue with ab70061 at 1/50 dilution, with and without the immunising peptide.
ab70061 has not yet been referenced specifically in any publications.