Anti-ROR alpha/RORA antibody (ab70061)
Key features and details
- Rabbit polyclonal to ROR alpha/RORA
- Suitable for: WB, IHC-P
- Reacts with: Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-ROR alpha/RORA antibody
See all ROR alpha/RORA primary antibodies -
Description
Rabbit polyclonal to ROR alpha/RORA -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, African green monkey -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human breast carcinoma tissue, extracts from HeLa and COS-7 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70061 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
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IHC-P |
1/50 - 1/100.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa). |
IHC-P
1/50 - 1/100. |
Target
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Function
Orphan nuclear receptor. Binds DNA as a monomer to hormone response elements (HRE) containing a single core motif half-site preceded by a short A-T-rich sequence. This isomer binds to the consensus sequence 5'-[AT][TA]A[AT][CGT]TAGGTCA-3'. Regulates a number of genes involved in lipid metabolism such as apolipoproteins AI, APOA5, CIII, CYP71 and PPARgamma, in cerebellum and photoreceptor development including PCP2, OPN1SW, OPN1SM AND ARR3, in circadian rhythm with BMAL1, and skeletal muscle development with MYOD1. Possible receptor for cholesterol or one of its derivatives. -
Tissue specificity
Widely expressed in a number of tissues. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Post-translational
modificationsPhosphorylation by PKC in neurons inhibits transcriptional activity. Phosphorylated on Thr-216 by ERK2 in vitro.
Sumoylated by SENP1 and SENP2. Sumoylation, promoted by PIAS2, PIAS3, PIASy but not PIAS1, enhances the transcriptional activity. Desumoylated by SENP1.
Ubiquitinated. Ubiquitination is required for efficient transcriptional activity and is prevented by Hairless. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6095 Human
- Omim: 600825 Human
- SwissProt: P35398 Human
- Unigene: 560343 Human
- Unigene: 655155 Human
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Alternative names
- fhl2a antibody
- NR1F1 antibody
- Nuclear receptor ROR alpha antibody
see all
Images
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All lanes : Anti-ROR alpha/RORA antibody (ab70061) at 1/500 dilution
Lane 1 : extract from HeLa cells
Lane 2 : extract from COS-7 cells
Lane 3 : extract from COS-7 cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 63 kDa
Observed band size: 63 kDa -
Human breast carcinoma tissue with ab70061 at 1/50 dilution, with and without the immunising peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab70061 has been referenced in 1 publication.
- Kang X et al. Sevoflurane blocks glioma malignant development by upregulating circRELN through circRELN-mediated miR-1290/RORA axis. BMC Anesthesiol 21:213 (2021). PubMed: 34479497