Recombinant Anti-ROR alpha/RORA antibody [EPR23719-18] (ab256799)
![Western blot - Anti-ROR alpha/RORA antibody [EPR23719-18] (ab256799)](https://www.abcam.com/ps/products/256/ab256799/Images/ab256799-448943-anti-ror-alpharora-antibody-epr2371918-western-blot-lysate.jpg "Western blot - Anti-ROR alpha/RORA antibody [EPR23719-18] (ab256799)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23719-18] to ROR alpha/RORA
- Suitable for: IP, IHC-P, WB
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-ROR alpha/RORA antibody [EPR23719-18]
See all ROR alpha/RORA primary antibodies -
Description
Rabbit monoclonal [EPR23719-18] to ROR alpha/RORA -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details
Unsuitable for: IHC-Fr -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment within Human ROR alpha/RORA aa 250 to the C-terminus. The exact sequence is proprietary.
Database link: P35398 -
Positive control
- WB: Mouse and rat cerebellum tissue lysate. IHC-P: Mouse and rat cerebellum tissue. IP: Mouse and rat cerebellum tissue lysate.
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General notes
This product is produced using clone 18 of EPR23719.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23719-18 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab256799 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | 1/30. | |
| IHC-P | 1/800. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| WB | 1/1000. Predicted molecular weight: 63 kDa. |
Target
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Function
Orphan nuclear receptor. Binds DNA as a monomer to hormone response elements (HRE) containing a single core motif half-site preceded by a short A-T-rich sequence. This isomer binds to the consensus sequence 5'-[AT][TA]A[AT][CGT]TAGGTCA-3'. Regulates a number of genes involved in lipid metabolism such as apolipoproteins AI, APOA5, CIII, CYP71 and PPARgamma, in cerebellum and photoreceptor development including PCP2, OPN1SW, OPN1SM AND ARR3, in circadian rhythm with BMAL1, and skeletal muscle development with MYOD1. Possible receptor for cholesterol or one of its derivatives. -
Tissue specificity
Widely expressed in a number of tissues. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Post-translational
modificationsPhosphorylation by PKC in neurons inhibits transcriptional activity. Phosphorylated on Thr-216 by ERK2 in vitro.
Sumoylated by SENP1 and SENP2. Sumoylation, promoted by PIAS2, PIAS3, PIASy but not PIAS1, enhances the transcriptional activity. Desumoylated by SENP1.
Ubiquitinated. Ubiquitination is required for efficient transcriptional activity and is prevented by Hairless. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 19883 Mouse
- Entrez Gene: 300807 Rat
- SwissProt: P51448 Mouse
- Unigene: 378450 Mouse
- Unigene: 391890 Mouse
- Unigene: 427266 Mouse
- Unigene: 490614 Mouse
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Alternative names
- fhl2a antibody
- NR1F1 antibody
- Nuclear receptor ROR alpha antibody
see all
Images
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Western blot - Anti-ROR alpha/RORA antibody [EPR23719-18] (ab256799)All lanes : Anti-ROR alpha/RORA antibody [EPR23719-18] (ab256799) at 1/1000 dilution
Lane 1 : Mouse cerebellum tissue lysate
Lane 2 : Mouse spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 63 kDa
Observed band size: 60, 65 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: mouse spleen (PMID:9226375).
The molecular weight observed is consistent with what has been described in the literature (PMID:19955433, PMID:12023888)
Exposure time: 3 minutes
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR alpha/RORA antibody [EPR23719-18] (ab256799)](https://www.abcam.com/ps/products/256/ab256799/Images/ab256799-1-anti-ror-alpharora-antibody-epr2371918-immunohistochemistry-mouse-cerebellum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR alpha/RORA antibody [EPR23719-18] (ab256799)Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling ROR alpha/RORA with ab256799 at 1/800 (0.601 µg/ml) dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse cerebellum (PMID:14966198). The section was incubated with ab256799 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Immunoprecipitation - Anti-ROR alpha/RORA antibody [EPR23719-18] (ab256799)](https://www.abcam.com/ps/products/256/ab256799/Images/ab256799-3-anti-ror-alpharora-antibody-epr2371918-immunoprecipitation-mouse-cerebellum-tissue-lysate-10ug.jpg)
Immunoprecipitation - Anti-ROR alpha/RORA antibody [EPR23719-18] (ab256799)ROR alpha/RORA was immunoprecipitated from 0.35 mg mouse cerebellum tissue lysate with ab256799 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256799 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse cerebellum tissue lysate 10µg
Lane 2: ab256799 IP in mouse cerebellum tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab256799 in mouse cerebellum tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Western blot - Anti-ROR alpha/RORA antibody [EPR23719-18] (ab256799)](https://www.abcam.com/ps/products/256/ab256799/Images/ab256799-448945-anti-ror-alpharora-antibody-epr2371918-western-blot-lysate1.jpg)
Western blot - Anti-ROR alpha/RORA antibody [EPR23719-18] (ab256799)Anti-ROR alpha/RORA antibody [EPR23719-18] (ab256799) at 1/1000 dilution + Rat cerebellum tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 63 kDa
Observed band size: 60, 65 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 3 minutes
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR alpha/RORA antibody [EPR23719-18] (ab256799)](https://www.abcam.com/ps/products/256/ab256799/Images/ab256799-2-anti-ror-alpharora-antibody-epr2371918-immunohistochemistry-rat-cerebellum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR alpha/RORA antibody [EPR23719-18] (ab256799)Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling ROR alpha/RORA with ab256799 at 1/800 (0.601 µg/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on rat cerebellum. The section was incubated with ab256799 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunoprecipitation - Anti-ROR alpha/RORA antibody [EPR23719-18] (ab256799)](https://www.abcam.com/ps/products/256/ab256799/Images/ab256799-4-anti-ror-alpharora-antibody-epr2371918-immunoprecipitation-rat-cerebellum-tissue-lysate-10ug.jpg)
Immunoprecipitation - Anti-ROR alpha/RORA antibody [EPR23719-18] (ab256799)ROR alpha/RORA was immunoprecipitated from 0.35 mg rat cerebellum tissue lysate with ab256799 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256799 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Rat cerebellum tissue lysate 10µg
Lane 2: ab256799 IP in rat cerebellum tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab256799 in rat cerebellum tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab256799 has not yet been referenced specifically in any publications.
