RecombinantRabMAb

Recombinant Anti-ROR alpha/RORA antibody [EPR23719-48] - BSA and Azide free (ab278109)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR alpha/RORA antibody [EPR23719-48] (ab278109)
  • Immunoprecipitation - Anti-ROR alpha/RORA antibody [EPR23719-48] (ab278109)
  • Immunohistochemistry (Frozen sections) - Anti-ROR alpha/RORA antibody [EPR23719-48] (ab278109)
  • Immunoprecipitation - Anti-ROR alpha/RORA antibody [EPR23719-48] (ab278109)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR alpha/RORA antibody [EPR23719-48] (ab278109)
  • Immunohistochemistry (Frozen sections) - Anti-ROR alpha/RORA antibody [EPR23719-48] (ab278109)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23719-48] to ROR alpha/RORA - BSA and Azide free
  • Suitable for: IP, IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-ROR alpha/RORA antibody [EPR23719-48] - BSA and Azide free
    See all ROR alpha/RORA primary antibodies

  • Description

    Rabbit monoclonal [EPR23719-48] to ROR alpha/RORA - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, IHC-P, IHC-Frmore details
    Unsuitable for: WB

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-Fr: Mouse Cerebellum tissue IHC-P: Mouse Cerebellum tissue; Rat Cerebellum tissue. IP: Mouse cerebellum tissue lysate; Rat cerebellum tissue lysate.

  • General notes

    ab278109 is the carrier-free version of ab278099.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab278109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
Use at an assay dependent concentration.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)
Notes
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
Use at an assay dependent concentration.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)
Application notes
Is unsuitable for WB.

Target

  • Function

    Orphan nuclear receptor. Binds DNA as a monomer to hormone response elements (HRE) containing a single core motif half-site preceded by a short A-T-rich sequence. This isomer binds to the consensus sequence 5'-[AT][TA]A[AT][CGT]TAGGTCA-3'. Regulates a number of genes involved in lipid metabolism such as apolipoproteins AI, APOA5, CIII, CYP71 and PPARgamma, in cerebellum and photoreceptor development including PCP2, OPN1SW, OPN1SM AND ARR3, in circadian rhythm with BMAL1, and skeletal muscle development with MYOD1. Possible receptor for cholesterol or one of its derivatives.

  • Tissue specificity

    Widely expressed in a number of tissues.

  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.

  • Post-translational
    modifications

    Phosphorylation by PKC in neurons inhibits transcriptional activity. Phosphorylated on Thr-216 by ERK2 in vitro.
    Sumoylated by SENP1 and SENP2. Sumoylation, promoted by PIAS2, PIAS3, PIASy but not PIAS1, enhances the transcriptional activity. Desumoylated by SENP1.
    Ubiquitinated. Ubiquitination is required for efficient transcriptional activity and is prevented by Hairless.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • fhl2a antibody
      • NR1F1 antibody
      • Nuclear receptor ROR alpha antibody
      • Nuclear receptor ROR-alpha antibody
      • Nuclear receptor RZR-alpha antibody
      • Nuclear receptor subfamily 1 group F member 1 antibody
      • RAR related orphan receptor A antibody
      • RAR related orphan receptor alpha antibody
      • RAR-related orphan receptor A antibody
      • Retinoid-related orphan receptor-alpha antibody
      • Rora antibody
      • RORA_HUMAN antibody
      • RZR alpha antibody
      • RZR-ALPHA antibody
      • RZRA antibody
      • Transcription factor RZR alpha antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR alpha/RORA antibody [EPR23719-48] (ab278109)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR alpha/RORA antibody [EPR23719-48] (ab278109)

      This data was developed using ab278099, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling ROR alpha/RORA with ab278099 at 1/800 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse cerebellum ( PMID:14966198). The section was incubated with ab278099 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Immunoprecipitation - Anti-ROR alpha/RORA antibody [EPR23719-48] (ab278109)
      Immunoprecipitation - Anti-ROR alpha/RORA antibody [EPR23719-48] (ab278109)

      This data was developed using ab278099, the same antibody clone in a different buffer formulation.

      ROR alpha/RORA was immunoprecipitated from 0.35 mg Mouse cerebellum tissue lysate with ab278099 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab278099 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: Mouse cerebellum tissue lysate 10ug

      Lane 2: ab278099 IP in Mouse cerebellum tissue lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab278099 in mouse cerebellum tissue lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 minutes

    • Immunohistochemistry (Frozen sections) - Anti-ROR alpha/RORA antibody [EPR23719-48] (ab278109)
      Immunohistochemistry (Frozen sections) - Anti-ROR alpha/RORA antibody [EPR23719-48] (ab278109)

      This data was developed using ab278099, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum tissue labeling ROR alpha/RORA with ab278099 at 1/50 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse cerebellum is observed. The nuclear counterstain was DAPI (Blue).

      Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    • Immunoprecipitation - Anti-ROR alpha/RORA antibody [EPR23719-48] (ab278109)
      Immunoprecipitation - Anti-ROR alpha/RORA antibody [EPR23719-48] (ab278109)

      This data was developed using ab278099, the same antibody clone in a different buffer formulation.

      ROR alpha/RORA was immunoprecipitated from 0.35 mg Rat cerebellum tissue lysate with ab278099 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab278099 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: Rat cerebellum tissue lysate 10ug

      Lane 2: ab278099 IP in Rat cerebellum tissue lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab278099 in rat cerebellum tissue lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 minutes

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR alpha/RORA antibody [EPR23719-48] (ab278109)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR alpha/RORA antibody [EPR23719-48] (ab278109)

      This data was developed using ab278099, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Rat cerebellum tissue labeling ROR alpha/RORA with ab278099 at 1/800 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on rat cerebellum. The section was incubated with ab278099 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

    • Immunohistochemistry (Frozen sections) - Anti-ROR alpha/RORA antibody [EPR23719-48] (ab278109)
      Immunohistochemistry (Frozen sections) - Anti-ROR alpha/RORA antibody [EPR23719-48] (ab278109)

      This data was developed using ab278099, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney tissue labeling ROR alpha/RORA with ab278099 at 1/50 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Negative control: no staining on mouse kidney (PMID: 9226375) is observed. The nuclear counterstain was DAPI (Blue).

      Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab278109? Please let us know so that we can cite the reference in this datasheet.

    ab278109 has not yet been referenced specifically in any publications.

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