Anti-ROR gamma antibody [162C2a] (ab58670)
Key features and details
- Mouse monoclonal [162C2a] to ROR gamma
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG2a
Overview
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Product name
Anti-ROR gamma antibody [162C2a]
See all ROR gamma primary antibodies -
Description
Mouse monoclonal [162C2a] to ROR gamma -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Recombinant fragment of Human ROR gamma
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.0225% Potassium chloride, 0.03% Potassium phosphate, 0.1312% Sodium phosphate, 0.812% Sodium chloride, 1% BSA -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
ab58670 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane. -
Clonality
Monoclonal -
Clone number
162C2a -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab58670 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 35 kDa (predicted molecular weight: 58 kDa).
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 35 kDa (predicted molecular weight: 58 kDa). |
Target
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Function
Nuclear receptor that binds DNA as a monomer to ROR response elements (RORE) containing a single core motif half-site 5'-AGGTCA-3' preceded by a short A-T-rich sequence. Key regulator of cellular differentiation, immunity, peripheral circadian rhythm as well as lipid, steroid, xenobiotics and glucose metabolism (PubMed:19381306, PubMed:19965867, PubMed:22789990, PubMed:26160376, PubMed:20203100). Considered to have intrinsic transcriptional activity, have some natural ligands like oxysterols that act as agonists (25-hydroxycholesterol) or inverse agonists (7-oxygenated sterols), enhancing or repressing the transcriptional activity, respectively (PubMed:19965867, PubMed:22789990). Recruits distinct combinations of cofactors to target gene regulatory regions to modulate their transcriptional expression, depending on the tissue, time and promoter contexts. Regulates the circadian expression of clock genes such as CRY1, ARNTL/BMAL1 and NR1D1 in peripheral tissues and in a tissue-selective manner. Competes with NR1D1 for binding to their shared DNA response element on some clock genes such as ARNTL/BMAL1, CRY1 and NR1D1 itself, resulting in NR1D1-mediated repression or RORC-mediated activation of the expression, leading to the circadian pattern of clock genes expression. Therefore influences the period length and stability of the clock. Involved in the regulation of the rhythmic expression of genes involved in glucose and lipid metabolism, including PLIN2 and AVPR1A (PubMed:19965867). Negative regulator of adipocyte differentiation through the regulation of early phase genes expression, such as MMP3. Controls adipogenesis as well as adipocyte size and modulates insulin sensitivity in obesity. In liver, has specific and redundant functions with RORA as positive or negative modulator of expression of genes encoding phase I and Phase II proteins involved in the metabolism of lipids, steroids and xenobiotics, such as SULT1E1. Also plays also a role in the regulation of hepatocyte glucose metabolism through the regulation of G6PC and PCK1 (PubMed:19965867). Regulates the rhythmic expression of PROX1 and promotes its nuclear localization (PubMed:19381306, PubMed:19965867, PubMed:22789990, PubMed:26160376, PubMed:20203100). Plays an indispensable role in the induction of IFN-gamma dependent anti-mycobacterial systemic immunity (PubMed:26160376).
Isoform 2: Essential for thymopoiesis and the development of several secondary lymphoid tissues, including lymph nodes and Peyer's patches. Required for the generation of LTi (lymphoid tissue inducer) cells. Regulates thymocyte survival through DNA-binding on ROREs of target gene promoter regions and recruitment of coactivaros via the AF-2. Also plays a key role, downstream of IL6 and TGFB and synergistically with RORA, for lineage specification of uncommitted CD4(+) T-helper (T(H)) cells into T(H)17 cells, antagonizing the T(H)1 program. Probably regulates IL17 and IL17F expression on T(H) by binding to the essential enhancer conserved non-coding sequence 2 (CNS2) in the IL17-IL17F locus. May also play a role in the pre-TCR activation cascade leading to the maturation of alpha/beta T-cells and may participate in the regulation of DNA accessibility in the TCR-J(alpha) locus. -
Tissue specificity
Isoform 1 is widely expressed in many tissues, including liver and adipose, and highly expressed in skeletal muscle. Isoform 2 is primarily expressed in immature thymocytes. -
Involvement in disease
Immunodeficiency 42 -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Domain
The AF-2 (activation function-2) motif is required for recruiting coregulators containing LXXLL motifs such as NCOA1 and NCOA2. -
Cellular localization
Nucleus. - Information by UniProt
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Alternative names
- IMD42 antibody
- MGC129539 antibody
- NR1F3 antibody
see all
Images
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Anti-ROR gamma antibody [162C2a] (ab58670) + immunising recombinant protein
Predicted band size: 58 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (5)
ab58670 has been referenced in 5 publications.
- Pastwińska J et al. Identification of Corosolic and Oleanolic Acids as Molecules Antagonizing the Human RORγT Nuclear Receptor Using the Calculated Fingerprints of the Molecular Similarity. Int J Mol Sci 23:N/A (2022). PubMed: 35163824
- Karas K et al. The Dichotomous Nature of AZ5104 (an EGFR Inhibitor) Towards ROR? and ROR?T. Int J Mol Sci 20:N/A (2019). PubMed: 31744223
- Karas K et al. The cardenolides strophanthidin, digoxigenin and dihydroouabain act as activators of the human ROR?/ROR?T receptors. Toxicol Lett 295:314-324 (2018). PubMed: 29981919
- Karas K et al. Digoxin, an Overlooked Agonist of ROR?/ROR?T. Front Pharmacol 9:1460 (2018). PubMed: 30666196
- Ratajewski M et al. Expression of human gene coding ROR?T receptor depends on the Sp2 transcription factor. J Leukoc Biol 100:1213-1223 (2016). PubMed: 27256574