Anti-ROR gamma antibody [4G419] (Phycoerythrin) (ab104950)


  • Product name

    Anti-ROR gamma antibody [4G419] (Phycoerythrin)
    See all ROR gamma primary antibodies
  • Description

    Mouse monoclonal [4G419] to ROR gamma (Phycoerythrin)
  • Host species

  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Specificity

    ab104950 will detect ROR gamma and may detect ROR gamma t, but can not be used to distinguish between the two proteins.
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human, Chimpanzee
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    A region within amino acids 1-50 of Human ROR gamma.

  • Positive control

    • Human lymphocytes



Our Abpromise guarantee covers the use of ab104950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells.





ab91357 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.



  • Function

    Nuclear receptor that binds DNA as a monomer to ROR response elements (RORE) containing a single core motif half-site 5'-AGGTCA-3' preceded by a short A-T-rich sequence. Key regulator of cellular differentiation, immunity, peripheral circadian rhythm as well as lipid, steroid, xenobiotics and glucose metabolism (PubMed:19381306, PubMed:19965867, PubMed:22789990, PubMed:26160376, PubMed:20203100). Considered to have intrinsic transcriptional activity, have some natural ligands like oxysterols that act as agonists (25-hydroxycholesterol) or inverse agonists (7-oxygenated sterols), enhancing or repressing the transcriptional activity, respectively (PubMed:19965867, PubMed:22789990). Recruits distinct combinations of cofactors to target gene regulatory regions to modulate their transcriptional expression, depending on the tissue, time and promoter contexts. Regulates the circadian expression of clock genes such as CRY1, ARNTL/BMAL1 and NR1D1 in peripheral tissues and in a tissue-selective manner. Competes with NR1D1 for binding to their shared DNA response element on some clock genes such as ARNTL/BMAL1, CRY1 and NR1D1 itself, resulting in NR1D1-mediated repression or RORC-mediated activation of the expression, leading to the circadian pattern of clock genes expression. Therefore influences the period length and stability of the clock. Involved in the regulation of the rhythmic expression of genes involved in glucose and lipid metabolism, including PLIN2 and AVPR1A (PubMed:19965867). Negative regulator of adipocyte differentiation through the regulation of early phase genes expression, such as MMP3. Controls adipogenesis as well as adipocyte size and modulates insulin sensitivity in obesity. In liver, has specific and redundant functions with RORA as positive or negative modulator of expression of genes encoding phase I and Phase II proteins involved in the metabolism of lipids, steroids and xenobiotics, such as SULT1E1. Also plays also a role in the regulation of hepatocyte glucose metabolism through the regulation of G6PC and PCK1 (PubMed:19965867). Regulates the rhythmic expression of PROX1 and promotes its nuclear localization (PubMed:19381306, PubMed:19965867, PubMed:22789990, PubMed:26160376, PubMed:20203100). Plays an indispensable role in the induction of IFN-gamma dependent anti-mycobacterial systemic immunity (PubMed:26160376).
    Isoform 2: Essential for thymopoiesis and the development of several secondary lymphoid tissues, including lymph nodes and Peyer's patches. Required for the generation of LTi (lymphoid tissue inducer) cells. Regulates thymocyte survival through DNA-binding on ROREs of target gene promoter regions and recruitment of coactivaros via the AF-2. Also plays a key role, downstream of IL6 and TGFB and synergistically with RORA, for lineage specification of uncommitted CD4(+) T-helper (T(H)) cells into T(H)17 cells, antagonizing the T(H)1 program. Probably regulates IL17 and IL17F expression on T(H) by binding to the essential enhancer conserved non-coding sequence 2 (CNS2) in the IL17-IL17F locus. May also play a role in the pre-TCR activation cascade leading to the maturation of alpha/beta T-cells and may participate in the regulation of DNA accessibility in the TCR-J(alpha) locus.
  • Tissue specificity

    Isoform 1 is widely expressed in many tissues, including liver and adipose, and highly expressed in skeletal muscle. Isoform 2 is primarily expressed in immature thymocytes.
  • Involvement in disease

    Immunodeficiency 42
  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain

    The AF-2 (activation function-2) motif is required for recruiting coregulators containing LXXLL motifs such as NCOA1 and NCOA2.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • IMD42 antibody
    • MGC129539 antibody
    • NR1F3 antibody
    • Nuclear receptor ROR gamma antibody
    • Nuclear receptor ROR-gamma antibody
    • Nuclear receptor RZR gamma antibody
    • Nuclear receptor RZR-gamma antibody
    • Nuclear receptor subfamily 1 group F member 3 antibody
    • RAR related orphan nuclear receptor variant 2 antibody
    • RAR related orphan receptor C antibody
    • RAR related orphan receptor C, isoform a antibody
    • RAR related orphan receptor gamma antibody
    • RAR-related orphan receptor C antibody
    • Retinoic acid binding receptor gamma antibody
    • Retinoid related orphan receptor gamma antibody
    • Retinoid-related orphan receptor-gamma antibody
    • Rorc antibody
    • RORG antibody
    • RORG_HUMAN antibody
    • RZR GAMMA antibody
    • RZRG antibody
    • TOR antibody
    see all


  • ab104950 at 10 µl per 1x106 cells (0.25 ug) staining ROR gamma in Human lymphocytes by Intracellular flow cytometric analysis. Shaded histogram represents cells without antibody; green represents isotype control; red represents ab104950.


ab104950 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Flow Cytometry
Rat Cell (Spleen)
Yes - eBiosciences Cat. No. 00-5523
Gating Strategy
Gating was performed on viable splenocytes (R1)
Cell harvesting/tissue preparation method: Rat splenocytes were isolated and erythrocytes were lyses by hypotonic lysis (Aqua Bidest + 2x PBS). Splenocytes were activated for 48 h by using Phytohaemagglutinin (PHA) 5 µg/ml. Splenocytes were washed in PBS (300 g, 10 min) and adjusted to 1x10^7 cells/ml in PBS + 2% FCS. 100µl cell suspension (1x10^6 cells) were stained with 10 µl (0.25 ug) ab104950 ROR gamma and analyzed by flow cytometry. Double staining was performed by using 0.5 µl (0.25 µg) CD4 FITC (eBioscience, 11-0040).
Sample buffer: Cells were washed, blocked and stained in PBS+2% FCS. For intracellular staining, a Transcription Factor Staining Buffer was used (eBiosciences Cat. No. 00-5523).
eBiosciences Cat. No. 00-5523

Abcam user community

Verified customer

Submitted Nov 04 2015


Thank you for your reply. I can confirm this antibody was tested on human lymphocytes in flow cytometry as shown in the data on the data sheet. I am sorry the antibody has regrettably not yet been tested on PMA/Ionomycin stimulated PBMC in flow cytometry. I would like to reassure you that all our products, including ab104950, are covered by our Abpromise guarantee in the tested applications and species listed on the datasheet. This means we are pleased to provide a refund, credit note or free of charge replacement if ab104950 is not working in the tested applications and species, including flow cytometry. The guarantee period is for 6 months after purchase. I am sorry we do not have the exact data you requested on this occasion. However, I hope the Abcam guarantee will provide some reassurances to you. If you have any further questions, please do not hesitate to contact us.

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