Key features and details
- Mouse monoclonal [2H6] to ROR1
- Suitable for: Flow Cyt, WB, ELISA, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-ROR1 antibody [2H6]
See all ROR1 primary antibodies
DescriptionMouse monoclonal [2H6] to ROR1
Tested applicationsSuitable for: Flow Cyt, WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment, corresponding to extracellular amino acids 30-406 of Human ROR1 fused with hIgGFc tag, expressed in HEK293 cells.
- Recombinant extracellular fragment (aa30-406) of Human ROR1 and HEK293 cells transfected with extracellular ROR1 (aa30-406)-hIgGFc.
This product was changed from ascites to supernatant. Lot no’s high than GR218279-13 are from Tissue Culture Supernatant
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab91187 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|WB||1/500 - 1/2000. Predicted molecular weight: 104 kDa.|
|ICC/IF||1/200 - 1/1000.|
FunctionTyrosine-protein kinase receptor whose role is not yet clear.
Tissue specificityExpressed strongly in human heart, lung and kidney, but weakly in the CNS. Isoform Short is strongly expressed in fetal and adult CNS and in a variety of human cancers, including those originating from CNS or PNS neuroectoderm.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. ROR subfamily.
Contains 1 FZ (frizzled) domain.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 kringle domain.
Contains 1 protein kinase domain.
Developmental stageExpressed at high levels during early embryonic development. The expression levels drop strongly around day 16 and there are only very low levels in adult tissues.
- Information by UniProt
- dJ537F10.1 antibody
- Inactive tyrosine protein kinase transmembrane receptor ROR1 antibody
- MGC99659 antibody
Anti-ROR1 antibody [2H6] (ab91187) at 1/500 dilution + recombinant extracellular fragment (aa30-406) of Human ROR1
Predicted band size: 104 kDa
ab91187 at 1/200 dilution staining ROR1 in HEK293 cells transfected with extracellular ROR1 (aa30-406)-hIgGFc by Confocal Immunofluorescence (green). DRAQ5 fluorescent DNA dye (blue).
Overlay histogram showing A549 cells stained with ab91187 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab91187, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
ab91187 has been referenced in 1 publication.
- Weekes MP et al. Quantitative temporal viromics: an approach to investigate host-pathogen interaction. Cell 157:1460-72 (2014). Flow Cyt ; Human . PubMed: 24906157