Product nameAnti-ROR1 antibody
See all ROR1 primary antibodies
DescriptionRabbit polyclonal to ROR1
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Recombinant fragment corresponding to Human ROR1 aa 112-399.
YGSRLRIRNLDTTDTGYFQCVATNGKEVVSSTGVLFVKFGPPPTASPGYS DEYEEDGFCQPYRGIACARFIGNRTVYMESLHMQGEIENQITAAFTMIGT SSHLSDKCSQFAIPSLCHYAFPYCDETSSVPKPRDLCRDECEILENVLCQ TEYIFARSNPMILMRLKLPNCEDLPQPESPEAANCIRIGIPMADPINKNH KCYNSTGVDYRGTVSVTKSGRQCQPWNSQYPHTHTFTALRFPELNGGHSY CRNPGNQKEAPWCFTLDENFKSDLCDIPACDSKDSKEK
Database link: Q01973-1
- WB: PANC-1 whole cell lysate. HEK-293 cell lysate, transiently transfected with the ROR1 gene. IHC-P: Human lung carcinoma, heart and kidney tissue. Flow Cytometry: NCI-H460 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab135669 is purified through a protein A column, followed by peptide affinity purification.
Our Abpromise guarantee covers the use of ab135669 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/50 - 1/100. Predicted molecular weight: 104 kDa.|
|IHC-P||1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionTyrosine-protein kinase receptor whose role is not yet clear.
Tissue specificityExpressed strongly in human heart, lung and kidney, but weakly in the CNS. Isoform Short is strongly expressed in fetal and adult CNS and in a variety of human cancers, including those originating from CNS or PNS neuroectoderm.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. ROR subfamily.
Contains 1 FZ (frizzled) domain.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 kringle domain.
Contains 1 protein kinase domain.
Developmental stageExpressed at high levels during early embryonic development. The expression levels drop strongly around day 16 and there are only very low levels in adult tissues.
- Information by UniProt
- dJ537F10.1 antibody
- Inactive tyrosine protein kinase transmembrane receptor ROR1 antibody
- MGC99659 antibody
ab135669 staining ROR1 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with formaldehyde and blocked with 3% BSA for 30 minutes at room temperature; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. An undiluted biotin-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.
Flow cytometric analysis of NCI-H460 cells labeling ROR1 with ab135669 at 1/10 dilution (bottom histogram) or negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Anti-ROR1 antibody (ab135669) at 1/2000 dilution + PANC-1 (Human pancreatic epithelial carcinoma cell line) whole cell lysate at 20 µg
Goat Anti-Rabbit IgG, (H+L)-HRP at 1/10000 dilution
Predicted band size: 104 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-ROR1 antibody (ab135669) at 1/50 dilution
Lane 1 : Non-transfected HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : ROR1 gene transfected HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
Lysates/proteins at 2 µg per lane.
Predicted band size: 104 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung carcinoma tissue labelling ROR1 with ab135669 at 1/50 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.
ab135669 staining ROR1 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with formaldehyde and blocked with 3% serum for 30 minutes at room temperature; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. An undiluted biotin-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.
This product has been referenced in:
- Zhang R et al. Atractylenolide II reverses the influence of lncRNA XIST/miR-30a-5p/ROR1 axis on chemo-resistance of colorectal cancer cells. J Cell Mol Med 23:3151-3165 (2019). Read more (PubMed: 30907503) »
- Wang X et al. miR-30a inhibits epithelial-mesenchymal transition and metastasis in triple-negative breast cancer by targeting ROR1. Oncol Rep 39:2635-2643 (2018). Read more (PubMed: 29693179) »