Recombinant Anti-ROR2 antibody [EPR19980] (ab218105)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19980] to ROR2
- Suitable for: IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ROR2 antibody [EPR19980]
See all ROR2 primary antibodies -
Description
Rabbit monoclonal [EPR19980] to ROR2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human breast, breast cancer, pancreas, pancreatic cancer, bladder cancer and gastrointestinal stromal tumors tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19980 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab218105 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684) |
Notes |
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IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684) |
Target
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Function
Tyrosine-protein kinase receptor which may be involved in the early formation of the chondrocytes. It seems to be required for cartilage and growth plate development. Phosphorylates YWHAB, leading to induction of osteogenesis and bone formation. -
Involvement in disease
Brachydactyly B1
Robinow syndrome autosomal recessive -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. ROR subfamily.
Contains 1 FZ (frizzled) domain.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 kringle domain.
Contains 1 protein kinase domain. -
Developmental stage
Expressed at high levels during early embryonic development. The expression levels drop strongly around day 16 and there are only very low levels in adult tissues. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 4920 Human
- Omim: 602337 Human
- SwissProt: Q01974 Human
- Unigene: 644776 Human
- Unigene: 98255 Human
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Alternative names
- BDB antibody
- BDB1 antibody
- Brachydactyly type B antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded human breast tissue labeling ROR2 with ab218105 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Positive membranous staining on human breast stroma cells; negative staining on epithelium cells.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling ROR2 with ab218105 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Membranous staining on human breast cancer is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling ROR2 with ab218105 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Positive membranous staining on human pancreas blood vessels; negative staining on epithelium cell.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue labeling ROR2 with ab218105 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Membranous staining on human pancreatic cancer is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling ROR2 with ab218105 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Membranous staining on human bladder cancer is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human gastrointestinal stromal tumors labeling ROR2 with ab218105 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Cytoplasmic membrane staining on human gastrointestinal stromal tumors is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab218105 has been referenced in 1 publication.
- Babin L et al. De novo generation of the NPM-ALK fusion recapitulates the pleiotropic phenotypes of ALK+ ALCL pathogenesis and reveals the ROR2 receptor as target for tumor cells. Mol Cancer 21:65 (2022). PubMed: 35246138