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    ror2-antibody-nt-2535-2835-ab190145.pdf

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Signal Transduction Protein Phosphorylation Tyrosine Kinases Receptor Tyrosine Kinases
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Validated using a knockout cell line

Anti-ROR2 antibody [Nt 2535-2835] (ab190145)

  • Datasheet
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Western blot - Anti-ROR2 antibody [Nt 2535-2835] (ab190145)
  • Western blot - Anti-ROR2 antibody [Nt 2535-2835] (ab190145)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR2 antibody [Nt 2535-2835] (ab190145)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR2 antibody [Nt 2535-2835] (ab190145)

Key features and details

  • Mouse monoclonal [Nt 2535-2835] to ROR2
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Rat, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-ROR2 antibody [Nt 2535-2835]
    See all ROR2 primary antibodies
  • Description

    Mouse monoclonal [Nt 2535-2835] to ROR2
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Fusion protein corresponding to Mouse ROR2. Ror2-MBP (maltose binding protein) fusion of the mouse Ror2 protein (bases 2535-2835).

  • Positive control

    • IHC-P: FFPE human prostate tissue sections. IHC-P: FFPE Human Stomach
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Some batches contain 6.97% L-Arginine as a stabilizing agent. For lot-specific buffer information, please contact our Scientific Support team.
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    Nt 2535-2835
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • Structures
    • Bone
    • Stem Cells
    • Mesenchymal Stem Cells
    • Osteogenesis

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant human ROR2 protein (ab70614)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab190145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 105 kDa.
IHC-P
Use a concentration of 10 mg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 105 kDa.
IHC-P
Use a concentration of 10 mg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application notes
Is unsuitable for ICC/IF.

Target

  • Function

    Tyrosine-protein kinase receptor which may be involved in the early formation of the chondrocytes. It seems to be required for cartilage and growth plate development. Phosphorylates YWHAB, leading to induction of osteogenesis and bone formation.
  • Involvement in disease

    Brachydactyly B1
    Robinow syndrome autosomal recessive
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. ROR subfamily.
    Contains 1 FZ (frizzled) domain.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 kringle domain.
    Contains 1 protein kinase domain.
  • Developmental stage

    Expressed at high levels during early embryonic development. The expression levels drop strongly around day 16 and there are only very low levels in adult tissues.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession Q01974 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4920 Human
    • Entrez Gene: 26564 Mouse
    • Entrez Gene: 306782 Rat
    • Omim: 602337 Human
    • SwissProt: Q01974 Human
    • SwissProt: Q9Z138 Mouse
    • Unigene: 644776 Human
    • Unigene: 98255 Human
    • Unigene: 342774 Mouse
    see all
  • Alternative names

    • BDB antibody
    • BDB1 antibody
    • Brachydactyly type B antibody
    • EC 2.7.10.1 antibody
    • MGC163394 antibody
    • Neurotrophic tyrosine kinase antibody
    • Neurotrophic tyrosine kinase, receptor related 2 antibody
    • NTRKR2 antibody
    • Receptor tyrosine kinase-like orphan receptor 2 antibody
    • receptor-related 2 antibody
    • ROR2 antibody
    • ROR2_HUMAN antibody
    • Tyrosine protein kinase transmembrane receptor ROR2 antibody
    • Tyrosine-protein kinase transmembrane receptor Ror2 antibody
    see all

Images

  • Western blot - Anti-ROR2 antibody [Nt 2535-2835] (ab190145)
    Western blot - Anti-ROR2 antibody [Nt 2535-2835] (ab190145)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: ROR2 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: MCF7 whole cell lysate (20 µg)
    Lane 4: U20S whole cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab190145 observed at 125 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab190145 was shown to recognize ROR2 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when ROR2 knockout samples were examined. Wild-type and ROR2 knockout samples were subjected to SDS-PAGE. Ab190145 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10,000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) ab216777 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-ROR2 antibody [Nt 2535-2835] (ab190145)
    Western blot - Anti-ROR2 antibody [Nt 2535-2835] (ab190145)
    All lanes : Anti-ROR2 antibody [Nt 2535-2835] (ab190145) at 1 µg/ml

    Lane 1 : Kidney (Mouse) Tissue Lysate
    Lane 2 : Lung (Rat) Tissue Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 105 kDa
    Observed band size: 100 kDa why is the actual band size different from the predicted?


    Exposure time: 2 minutes
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR2 antibody [Nt 2535-2835] (ab190145)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR2 antibody [Nt 2535-2835] (ab190145)

    IHC image of ROR2 staining in Normal Human Stomach formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F/B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab190145, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR2 antibody [Nt 2535-2835] (ab190145)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR2 antibody [Nt 2535-2835] (ab190145)

    IHC image of ROR2 staining in Human normal prostate formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab190145, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (2)

Publishing research using ab190145? Please let us know so that we can cite the reference in this datasheet.

ab190145 has been referenced in 2 publications.

  • Yang K  et al. Whole-exome sequencing identified compound heterozygous variants in ROR2 gene in a fetus with Robinow syndrome. J Clin Lab Anal 34:e23074 (2020). PubMed: 31617258
  • Roarty K  et al. Ror2 regulates branching, differentiation, and actin-cytoskeletal dynamics within the mammary epithelium. J Cell Biol 208:351-66 (2015). PubMed: 25624393

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