Overview

  • Product name

    Anti-ROS1 antibody [EPMGHR2] - BSA and Azide free
    See all ROS1 primary antibodies
  • Description

    Rabbit monoclonal [EPMGHR2] to ROS1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ROS1 aa 2050-2150. The exact sequence is proprietary.
    Database link: P08922

  • Positive control

    • IHC-P: Human lung carcinoma tissue,
  • General notes

    Ab238986 is the carrier-free version of ab189925. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab238986 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab238986 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    This is probably a cell growth or differentiation factor receptor with a tyrosine-protein kinase activity.
  • Tissue specificity

    Expressed in brain. Expression is increased in primary gliomas.
  • Involvement in disease

    Note=A chromosomal aberration involving ROS1 is found in a glioblastoma multiforme sample. An intra-chromosomal deletion del(6)(q21q21) is responsible for the formation of GOPC-ROS1 chimeric protein which has a constitutive receptor tyrosine kinase activity.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 9 fibronectin type-III domains.
    Contains 1 protein kinase domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • c ros 1 antibody
    • c ros antibody
    • c ros oncogene 1 receptor tyrosine kinase antibody
    • c Ros receptor tyrosine kinase antibody
    • c ros1 antibody
    • MCF 3 antibody
    • MCF3 antibody
    • Oncogene ROS antibody
    • OTTHUMP00000017814 antibody
    • OTTHUMP00000017815 antibody
    • OTTHUMP00000040389 antibody
    • Proto oncogene c ros 1 protein antibody
    • Proto oncogene c Ros antibody
    • Proto oncogene tyrosine protein kinase ROS antibody
    • Proto oncogene tyrosine protein kinase ROS precursor antibody
    • Proto-oncogene c-Ros-1 antibody
    • Proto-oncogene tyrosine-protein kinase ROS antibody
    • Receptor tyrosine kinase c ros oncogene 1 antibody
    • Ros 1 antibody
    • ROS 1C antibody
    • ROS antibody
    • ROS proto oncogene 1 , receptor tyrosine kinase antibody
    • ROS_HUMAN antibody
    • ROS1 antibody
    • ROS1C antibody
    • Transmembrane tyrosine specific protein kinase antibody
    • v ros avian UR2 sarcoma virus oncogene homolog 1 antibody
    • v ros UR2 sarcoma virus oncogene homolog 1 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling ROS1 with unpurified ab189925 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin. (Inset: negative control).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab189925).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling ROS1 with Purified  ab189925 at 1:300 dilution (5.23 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab189925).

References

ab238986 has not yet been referenced specifically in any publications.

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