Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9625] to ROS1
- Suitable for: WB
- Reacts with: Rat, Human
Product nameAnti-ROS1 antibody [EPR9625]
See all ROS1 primary antibodies
DescriptionRabbit monoclonal [EPR9625] to ROS1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Rat, Human
within Human ROS1 aa 2250 to the C-terminus. The exact sequence is proprietary.
Database link: P08922
- Calu-3 and HepG2 cell lysates.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181113 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 264 kDa).|
FunctionThis is probably a cell growth or differentiation factor receptor with a tyrosine-protein kinase activity.
Tissue specificityExpressed in brain. Expression is increased in primary gliomas.
Involvement in diseaseNote=A chromosomal aberration involving ROS1 is found in a glioblastoma multiforme sample. An intra-chromosomal deletion del(6)(q21q21) is responsible for the formation of GOPC-ROS1 chimeric protein which has a constitutive receptor tyrosine kinase activity.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 9 fibronectin type-III domains.
Contains 1 protein kinase domain.
- Information by UniProt
- c ros 1 antibody
- c ros antibody
- c ros oncogene 1 receptor tyrosine kinase antibody
All lanes : Anti-ROS1 antibody [EPR9625] (ab181113) at 1/5000 dilution
Lane 1 : Calu-3 cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 264 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
70kDa band is a truncated form of ROS protein
ab181113 has not yet been referenced specifically in any publications.