Anti-Rotavirus antibody [A2] (ab181695)
Key features and details
- Mouse monoclonal [A2] to Rotavirus
- Suitable for: WB, ICC/IF
- Isotype: IgG2b
Overview
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Product name
Anti-Rotavirus antibody [A2]
See all Rotavirus primary antibodies -
Description
Mouse monoclonal [A2] to Rotavirus -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Other species -
Immunogen
Recombinant fragment corresponding to Rotavirus. Inner capside protein VP6.
Database link: P04509 -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 0.75% Glycine, 2% Sucrose, 1.21% Tris -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
A2 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab181695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/200 - 1/1000. Predicted molecular weight: 45 kDa. | |
| ICC/IF | 1/100 - 1/500. |
Target
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Relevance
Rotaviruses, members of the family Reoviridae, are a major cause of diarrhoea in young mammals. Rotavirus infections also result in economic losses in agriculture due to diarrhoea in calf, pig, sheep, and poultry rearing. Diarrhoea (or scours) due to the rotavirus Nebraska Calf Diarrhea Virus can affect calves up to 30 days of age or older. Diarrhoea begins 2 to 3 days after exposure. Diagnosis is by history, lesions (ulcers on the tongue, lips, and mouth) and diagnostic laboratory tests. Mortality rates may be as high as 50 percent, depending on the secondary bacteria present. Human rotaviruses, the major aetiological agents of severe infantile diarrhoea worldwide, display surprisingly diverse and complex serotypic specificities. Rotaviruses are 70 nm, non enveloped viruses comprised of a triple layered protein capsid; Outer capsid proteins are VP4 and VP7, Inner capsid -VP6 and Core -VP2. The immunity acquired from exposure to rotavirus appears to be type specific following initial infection; therefore, multiple serotypes of rotavirus mean multiple opportunities for infection. The combination of animal reservoirs for the virus and rotavirus gene reassortment provides the potential for dramatic genetic shifts (similar to influenza virus) which could give rise to altered host ranges and viral virulence. -
Database links
- Entrez Gene: 7011361 Other species
- SwissProt: A7J3A1 Other species
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Alternative names
- Major inner capsid protein VP6 antibody
- NSP4 antibody
- VP6 antibody
Protocols
References (4)
ab181695 has been referenced in 4 publications.
- Li Z et al. A milk-based self-assemble rotavirus VP6-ferritin nanoparticle vaccine elicited protection against the viral infection. J Nanobiotechnology 17:13 (2019). PubMed: 30670042
- Hakim MS et al. Basal interferon signaling and therapeutic use of interferons in controlling rotavirus infection in human intestinal cells and organoids. Sci Rep 8:8341 (2018). PubMed: 29844362
- Hakim MS et al. TNF-a exerts potent anti-rotavirus effects via the activation of classical NF-?B pathway. Virus Res 253:28-37 (2018). ICC/IF . PubMed: 29859235
- Gao J et al. MYH9 is an Essential Factor for Porcine Reproductive and Respiratory Syndrome Virus Infection. Sci Rep 6:25120 (2016). PubMed: 27112594
