Anti-Rotavirus antibody [A2] (ab181695)
Key features and details
- Mouse monoclonal [A2] to Rotavirus
- Suitable for: WB, ICC/IF
- Reacts with: Rotavirus
- Isotype: IgG2b
Overview
-
Product name
Anti-Rotavirus antibody [A2]
See all Rotavirus primary antibodies -
Description
Mouse monoclonal [A2] to Rotavirus -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Rotavirus -
Immunogen
Recombinant fragment corresponding to Rotavirus. Inner capside protein VP6.
Database link: P04509 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 0.75% Glycine, 2% Sucrose, 1.21% Tris -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
A2 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/200 - 1/1000. Predicted molecular weight: 45 kDa.
|
|
ICC/IF | (1) |
1/100 - 1/500.
|
Notes |
---|
WB
1/200 - 1/1000. Predicted molecular weight: 45 kDa. |
ICC/IF
1/100 - 1/500. |
Target
-
Relevance
Rotaviruses, members of the family Reoviridae, are a major cause of diarrhoea in young mammals. Rotavirus infections also result in economic losses in agriculture due to diarrhoea in calf, pig, sheep, and poultry rearing. Diarrhoea (or scours) due to the rotavirus Nebraska Calf Diarrhea Virus can affect calves up to 30 days of age or older. Diarrhoea begins 2 to 3 days after exposure. Diagnosis is by history, lesions (ulcers on the tongue, lips, and mouth) and diagnostic laboratory tests. Mortality rates may be as high as 50 percent, depending on the secondary bacteria present. Human rotaviruses, the major aetiological agents of severe infantile diarrhoea worldwide, display surprisingly diverse and complex serotypic specificities. Rotaviruses are 70 nm, non enveloped viruses comprised of a triple layered protein capsid; Outer capsid proteins are VP4 and VP7, Inner capsid -VP6 and Core -VP2. The immunity acquired from exposure to rotavirus appears to be type specific following initial infection; therefore, multiple serotypes of rotavirus mean multiple opportunities for infection. The combination of animal reservoirs for the virus and rotavirus gene reassortment provides the potential for dramatic genetic shifts (similar to influenza virus) which could give rise to altered host ranges and viral virulence. -
Alternative names
- Major inner capsid protein VP6 antibody
- NSP4 antibody
- VP6 antibody
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (6)
ab181695 has been referenced in 6 publications.
- Bruno C et al. Protective effects of the postbiotic deriving from cow's milk fermentation with L. paracasei CBA L74 against Rotavirus infection in human enterocytes. Sci Rep 12:6268 (2022). PubMed: 35428750
- Yamada K et al. Screening for Components/Compounds with Anti-Rotavirus Activity: Detection of Interaction Between Viral Spike Proteins and Glycans. Methods Mol Biol 2132:585-595 (2020). PubMed: 32306358
- Li Z et al. A milk-based self-assemble rotavirus VP6-ferritin nanoparticle vaccine elicited protection against the viral infection. J Nanobiotechnology 17:13 (2019). PubMed: 30670042
- Hakim MS et al. Basal interferon signaling and therapeutic use of interferons in controlling rotavirus infection in human intestinal cells and organoids. Sci Rep 8:8341 (2018). PubMed: 29844362
- Hakim MS et al. TNF-a exerts potent anti-rotavirus effects via the activation of classical NF-?B pathway. Virus Res 253:28-37 (2018). ICC/IF . PubMed: 29859235
- Gao J et al. MYH9 is an Essential Factor for Porcine Reproductive and Respiratory Syndrome Virus Infection. Sci Rep 6:25120 (2016). PubMed: 27112594