Key features and details
- Goat polyclonal to Rotavirus (FITC)
- Suitable for: ICC/IF, ELISA
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
Product nameAnti-Rotavirus antibody (FITC)
See all Rotavirus primary antibodies
DescriptionGoat polyclonal to Rotavirus (FITC)
ConjugationFITC. Ex: 493nm, Em: 528nm
Tested applicationsSuitable for: ICC/IF, ELISAmore details
Species reactivityReacts with: Other species
Tissue/ cell preparation: Bovine rotavirus (whole virus).
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
PurityIon Exchange Chromatography
Purification notesAmmonium sulfate fractionation and ion-exchange chromatography.
Our Abpromise guarantee covers the use of ab31435 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/250. Acetone fixed.|
|ELISA||Use at an assay dependent dilution.|
RelevanceRotaviruses, members of the family Reoviridae, are a major cause of diarrhoea in young mammals. Rotavirus infections also result in economic losses in agriculture due to diarrhoea in calf, pig, sheep, and poultry rearing. Diarrhoea (or scours) due to the rotavirus Nebraska Calf Diarrhea Virus can affect calves up to 30 days of age or older. Diarrhoea begins 2 to 3 days after exposure. Diagnosis is by history, lesions (ulcers on the tongue, lips, and mouth) and diagnostic laboratory tests. Mortality rates may be as high as 50 percent, depending on the secondary bacteria present. Human rotaviruses, the major aetiological agents of severe infantile diarrhoea worldwide, display surprisingly diverse and complex serotypic specificities. Rotaviruses are 70 nm, non enveloped viruses comprised of a triple layered protein capsid; Outer capsid proteins are VP4 and VP7, Inner capsid -VP6 and Core -VP2. The immunity acquired from exposure to rotavirus appears to be type specific following initial infection; therefore, multiple serotypes of rotavirus mean multiple opportunities for infection. The combination of animal reservoirs for the virus and rotavirus gene reassortment provides the potential for dramatic genetic shifts (similar to influenza virus) which could give rise to altered host ranges and viral virulence.
- Major inner capsid protein VP6 antibody
- NSP4 antibody
- VP6 antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab31435 has been referenced in 1 publication.
- Buccigrossi V et al. Mechanisms of antidiarrhoeal effects by diosmectite in human intestinal cells. Gut Pathog 9:23 (2017). PubMed: 28450899