Key features and details
- Rabbit polyclonal to ROX
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ROX antibody
See all ROX primary antibodies
DescriptionRabbit polyclonal to ROX
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human ROX (N terminal).
Database link: NP_694822
- Human heart tissue lysate; Mouse heart tissue.
This product was previously labelled as MINA53
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab126282 is affinity chromatography purified via peptide column.
Our Abpromise guarantee covers the use of ab126282 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 20 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 53 kDa.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.|
FunctionInvolved in cellular proliferation. May play an important role in cell growth and survival. May be involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles.
Tissue specificityExpressed in liver, skeletal muscle, heart, pancreas, and placenta. Not detected in brain, lung or kidney. Expressed in several lung cancer tissues, but is barely detected in the adjacent non-cancerous tissues. Also highly expressed in several esophageal squamous cell carcinoma (ESCC), and colon cancer tissues, and in various cancer cell lines.
Sequence similaritiesBelongs to the MINA53/NO66 family.
Contains 1 JmjC domain.
Cellular localizationNucleus. Nucleus > nucleolus.
- Information by UniProt
- 60S ribosomal protein L27a histidine hydroxylase antibody
- Bifunctional lysine specific demethylase and histidyl hydroxylase MINA antibody
- FLJ14393 antibody
Lane 1 : Anti-ROX antibody (ab126282) at 1 µg/ml
Lane 2 : Anti-ROX antibody (ab126282) at 2 µg/ml
All lanes : Human heart tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 53 kDa
Immunofluorescence of ROX in mouse heart tissue with ab126282 at 20 ug/mL.
ab126282, at 5 ug/ml, staining ROX in Paraffin-embedded Mouse heart tissue by Immunohistochemistry.
ab126282 has been referenced in 2 publications.
- Fujino S et al. Mina53 nuclear localization is an important indicator of prognosis in patients with colorectal cancer after adjuvant chemotherapy. Oncol Rep 40:101-110 (2018). PubMed: 29750310
- Bellut J et al. Differential prognostic value of MYC immunohistochemistry in subtypes of papillary renal cell carcinoma. Sci Rep 7:16424 (2017). PubMed: 29180625